Stratification methods for assessing the progression and risk of advanced colorectal adenoma and colorectal cancer
AssigneeSociete De Commercialisation Des Produits De La Recherche Appliquee Socpra Sante Et Humaines, S.E.C.
InventorsJean-francois BEAULIEU, Elizabeth Herring, Eric Tremblay
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Abstract
The present disclosure concerns a method for stratifying the risk of a subject of having an advanced colorectal adenoma or a colorectal cancer based on determining the presence of overexpressed mRNA transcripts in the subject's stool. The method can be used to screen for subjects suitable for a colonoscopy. The method can also be used to tailor the stratified subject's treatment regimen.
Claims33
A method of stratifying the risk of a subject of having an advanced colorectal adenoma or a colorectal cancer in a subject, the method comprises:
- a) providing a stool sample from the subject, wherein the stool sample comprises a plurality of mRNA transcripts from the subject;
- b) determining the mRNA expression level of at least two distinct genes from the plurality of mRNA transcripts to obtain a test expression profile;
- and
- c) comparing the test expression profile with a control expression profile, wherein the control expression profile comprises the mRNA expression level of the at least two genes and is derived from a plurality of control mRNA transcripts from a control subject known to lack the advanced colorectal adenoma or the colorectal cancer;
- wherein if it is determined that the test expression profile of the subject comprises at least two genes whose expression are increased with the respect to the control expression profile, the subject is stratified as having an increased risk of having the advanced colorectal adenoma or the colorectal cancer, when compared to the control subject.
A kit for stratifying the risk of a subject of having an advanced colorectal adenoma or a colorectal cancer in a subject, wherein the kit comprises at least two reagents for determining the mRNA expression level of at least two distinct genes from the plurality of mRNA transcripts to obtain a test expression profile in a stool sample from the subject.
Dependencies are inferred from the claim text.