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US 2023/0399698 A1Published application
Assays for detection of acute lyme disease
AssigneesThe Regents of the University of California, The Johns Hopkins University
InventorsCharles Y. CHIU, Jerome BOUQUET, Mark J. SOLOSKI, John N. AUCOTT
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Abstract
The present disclosure relates to measuring gene expression of cells of a blood sample obtained from a mammalian subject suspected of having a tick-borne disease. In particular, the present disclosure provides tools for determining whether a human subject has acute Lyme disease by transcriptome profiling a peripheral blood mononuclear cell sample from the subject.
Claims36
A method for measuring gene expression, comprising the steps of:
- (a) measuring RNA expression of a plurality of genes of cells from a blood sample obtained from a mammalian subject suspected of having a tick-borne disease;
- (b) calculating a weighted RNA expression score for each of the plurality of genes;
- and
- (c) calculating a Lyme disease score by taking the sum of the weighted RNA expression scores.
1 more element
A kit comprising:
- (a) a plurality of oligonucleotides which hybridize to a plurality of genes comprising at least 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19 or all 20 genes of the group consisting of ANXA5, C3orf14, CDCA2, CR1, GBP2, IFI27, ITGAM, KCNJ2, KIF4A, MLF1IP, NCF1, PLBD1, PLK1, RAD51, SLC25A37, STAB1, STEAP4, TBP, TNFSF13B, and ZNF276;
- and
- (b) instructions for:
- (i) use of the oligonucleotides for measuring RNA expression of the plurality of genes;
- (ii) calculating a weighted RNA expression score for each of the plurality of genes;
- and (iii) calculating a Lyme disease score by taking the sum of the weighted RNA expression scores.
3 more elements
Dependencies are inferred from the claim text.