Assessing risk with pre-operative total cell-free dna
OwnerAoy Tomita Mitchell, Michael Mitchellinventors
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Abstract
This invention relates to methods and compositions for assessing risk based on amount(s) of total cell-free DNA in a subject, such as including at least one pre-operative sample from the subject.
Claims40
A method of assessing a risk in a subject, the method comprising:
- (a) obtaining an amount of pre-operative total cell-free DNA (cf-DNA) in a sample from the subject;
- and
- (b) optionally, reporting and/or recording the amount of pre-operative total cf-DNA.
The method of any one of the preceding claims, wherein the method further comprises:
- (e) obtaining an amount of pre-operative total cf-DNA in one or more additional samples from the subject, each taken at different point in time.
The method of any one of the preceding claims, wherein the method further comprises:
- (h) obtaining an amount of post-operative total cf-DNA in one or more additional samples from the subject, each taken at different point in time.
The method of any one of the preceding claims, wherein the method further comprises:
- determining a treatment or monitoring regimen for the subject based on the amount(s) of pre-operative total cf-DNA and/or post-operative total cf-DNA and/or the comparison(s).
The method of any one of the preceding claims, wherein pre-operative total cf-DNA and/or post-operative total cf-DNA is obtained from samples taken from the subject once, twice, or thrice daily.
The method of any one of the preceding claims, wherein the subject is a pediatric subject.
The method of any one of the preceding claims, wherein the subject is assessed for at least 1, 2, 3, 4, 5, 6, or 7 days prior to surgery.
The method of any one of the preceding claims, wherein the method further comprises providing or obtaining one or more threshold values or amount(s) from one or more different points in time.
The method of any one of the preceding claims, wherein the method further comprises obtaining the sample(s) from the subject.
The method of any one of the preceding claims, wherein the amount(s) and/or threshold value(s) are provided in a report.
A report that comprises the amount(s) and/or threshold value(s) of any one of the preceding claims.
A database that comprises the amount(s) and/or threshold value(s) of any one of the preceding claims.
The method of any one of the preceding claims, wherein the time between samples is decreased if the amount(s) of pre-operative total cf-DNA and/or post-operative total cf-DNA is increased relative to threshold(s) or amount(s) from earlier time point(s).
The method of any one of the preceding claims, wherein the time between samples is increased if the amount of pre-operative total cf-DNA and/or post-operative total cf-DNA is decreased relative to threshold(s) or amount(s) from earlier time point(s).
The method of any one of the preceding claims, wherein the sample is a blood, plasma or serum sample.
The method of any one of the preceding claims, wherein the subject is a non-transplant subject.
The method of any one of the preceding claims, wherein the subject is a cardiac surgery subject.
The method of any one of the preceding claims, wherein the subject is any one of the subjects provided herein.
The method of any one of the preceding claims, wherein the risk is a risk of cardiac arrest, need for mechanical ventilation, or death following surgery.
The method of any one of the preceding claims, wherein the risk is of any one of the states or conditions or outcomes as provided herein.
The method of any one of the preceding claims, wherein the threshold(s) is/are any one of the threshold value(s) as provided herein, such as 15, 16, 17 (e.g., 17.1), 18, 19, or 20 ng/mL.
The method of any one of the preceding claims, wherein the subject is monitored over time, such as with any one of the monitoring regimens as provided herein.
The method of any one of the preceding claims, wherein the subject is treated or treated over time, such as with any one of the treatments provided herein.
The method of any one of the preceding claims, wherein the treatment or monitoring regimen is suggested to, prescribed for, given to or performed on the subject.
Dependencies are inferred from the claim text.