New polymerase and use thereof
OwnerOla S¿derberg, Leonie Wenson +3inventors
InventorsOla S¿derberg, Leonie Wenson, Bjõrn Hellman, Erik Bivehed, Johan Heldin
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Abstract
The disclosure relates to a recombinant DNA dependent DNA polymerase having 5′-3′ exonuclease activity and lacking 3′-5′ exonuclease activity, wherein said polymerase is capable of extending DNA polymerisation from a single mismatched base pair and has an error rate of at least 1:1000. The disclosure further relates to a nucleic acid molecule encoding the recombinant DNA dependent DNA polymerase, a method for synthesizing double stranded DNA (dsDNA), a method for obtaining the position of a single strand break in a template dsDNA molecule, a method to introduce mutations in DNA of bacterial or eukaryotic cells, or organisms.
Claims20
A recombinant DNA dependent DNA polymerase having 5′-3′ exonuclease activity and lacking 3′-5′ exonuclease activity, wherein said polymerase is capable of extending DNA polymerisation from a mismatched base pair and has an error rate of at least 1:1000.
Dependencies are inferred from the claim text.