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US 2023/0399680 A1Published application
Methods and kits for labeling cellular molecules
AssigneeUniversity of Washington
InventorsGeorg Seelig, Richard Muscat, Alexander B. Rosenberg
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Abstract
Methods of uniquely labeling or barcoding molecules within a cell, a plurality of cells, and/or a tissue are provided. Kits for uniquely labeling or barcoding molecules within a cell, a plurality of cells, and/or a tissue are also provided. The molecules to be labeled may include, but are not limited to, RNAs, cDNAs, DNAs, proteins, peptides, and/or antigens.
Claims24
A kit for labeling deoxyribonucleic acid (DNA) molecules in situ, the kit comprising:
- (a) a plurality of nucleic acid adapter molecules;
- (b) a transposase;
- (c) one or more sets of nucleic acid tags, wherein each of the nucleic acid tags within the one or more sets comprises a barcode sequence, and wherein multiple distinct barcode sequences are present among the nucleic acid tags of each set;
- (d) a ligation agent;
- (e) a lysis agent;
- (f) a plurality of amplification primers, wherein at least a portion of the plurality of amplification primers comprise an index sequence, and wherein multiple distinct index sequences are present among the plurality of amplification primers;
- and
- (g) a DNA polymerase suitable for polymerase chain reaction (PCR) amplification.
5 more elements
Dependencies are inferred from the claim text.