A method for single-cell nascent rna labeling and sequencing
OwnerTongtong SUN, Guoxin TANG +2inventors
InventorsTongtong SUN, Guoxin TANG, Ji LI, Jiameng SUN
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Abstract
The present disclosure provides a sequencing method for labeling a mass of newly born single cell mRNAs, including a method for labeling a mass of newly born single cell mRNAs (Dynamic-seq technology) and a method for preparing a SCOPE single-cell sequencing library. The present disclosure uses S4U to mark new RNA in single cells in large quantities, cleaves single cells and captures mRNA, and constructs the obtained total RNA in transcriptomic library after IAA treatment, so as to obtain the transcriptomic sequencing library of labeled single cells.
Claims7
A sequencing method for mass labeling of newly born mRNA of single cells, comprising:
- a step of labeling newly born mRNA of mass single cells;
- and a step of preparation of SCOPE single-cell sequencing library.
The method of claim 5, wherein the IAA reagent is added to the chip in ice, and 200 ul FC-40 is added to the chip in time.
The method of claim 6, wherein the chip is placed at room temperature and on ice for 10 minutes respectively after 15 min of reaction at 50° C., and 200 ul FC-40 is added into the chip to remove the bubbles.
Dependencies are inferred from the claim text.