Methods and kits for nucleic acid purification

A method for purifying nucleic acids from a sample containing nucleic acids to be purified, wherein the method comprises the following steps conducted in the order i)-iv):

• i) contacting the sample with a solid phase material having a glass surface in presence of a binding buffer containing a chaotropic salt to bind the nucleic acids to the glass surface and obtain a solid phase material having the nucleic acids bound to its glass surface;
• ii) washing the solid phase material having the nucleic acids bound to s glass surface with a first wash buffer containing a C2-C4 aliphatic alcohol;
• iii) washing the solid phase material having the nucleic acids bound to its glass surface with a final wash buffer comprising at least about 50 wt-% dimethyl sulfoxide;
• and after step iii)
• iv) eluting the nucleic acids from the glass surface with an elution buffer to provide purified nucleic acids.