Methods and compositions for degrading deoxynivalenol

The method according to any one of the preceding claims, said method further comprising:

• incubating said nutritive source or material with one or more of (a)-(b) under conditions suitable for degrading DON and/or said DON derivative/s and/or altering toxicity of DON and/or said DON derivative/s;
• preferably said method further comprising heat-treating and/or fractionating and/or drying the product of said incubation;
• further preferably said altering toxicity comprising one or more of the following:
• detoxifying, and/or intramolecularly rearranging DON and/or said DON derivative/s.

A method for degrading deoxynivalenol (DON) and/or DON derivative selected from the group consisting of:

• 3-keto Deoxynivalenol, 3-epi Deoxynivalenol, 15-Acetyldeoxynivalenol, Nivalenol, Fusarenon-X (4-acetylnivalenol), 3-amino Deoxynivalenol and/or altering toxicity of DON and/or said DON derivative/s, said method comprising:
• i) providing one or more of the following polypeptides, wherein said one or more polypeptides are capable of modifying the C7-atom of deoxynivalenol (DON) and/or DON derivative selected from the group consisting of:
• 3-keto Deoxynivalenol, 3-epi Deoxynivalenol, 15-Acetyldemrynivalenol, Nivalenol, Fusarenon-X (4-acetylnivalenol), 3-amino Deoxynivalenol:
• (a) a polypeptide comprising the amino acid sequence set forth in SEQ ID NO:
• 1;
• (b) a polypeptide comprising an amino acid sequence having at least 70% (e.g., at least 95%, at least 96%, at least 97%, at least 98% or at least 99%) identity to the amino acid sequence set forth in SEQ ID NO:
• 1, but less than 100% sequence identity with the amino acid sequence set forth in SEQ ID NOs:
• 2 or 3, wherein said polypeptide is capable of modifying the C7-atom of deoxynivalenol (DON) and/or DON derivative selected from the group consisting of:
• 3-keto Deoxynivalenol, 3-epi Deoxynivalenol, 15-Acetyldeoxynivalenol, Nivalenol, Fusarenon-X (4-acetylnivalenol), 3-amino Deoxynivalenol;
• ii) applying one or more of (a)-(b) to DON and/or said DON derivative/s;
• preferably said one or more polypeptides are one or more recombinant and/or isolated polypeptides;
• further preferably said altering toxicity comprising one or more of the following:
• detoxifying and/or intramolecularly rearranging DON and/or DON derivative/s;
• most preferably said one or more polypeptides are capable of modifying the C7-atom of DON and/or DON derivative/s.

A composition or kit, comprising:

• (i) a polypeptide capable of degrading deoxynivalenol (DON) and/or DON derivative selected from the group consisting of:
• 3-keto Deoxynivalenol, 3-epi Deoxynivalenol, 15-Acetyldeoxynivalenol, Nivalenol, Fusarenon-X (4-acetylnivalenol), 3-amino Deoxynivalenol and/or altering toxicity of DON and/or said DON derivative/s, wherein said polypeptide is one or more of the following:
• (a) a polypeptide comprising the amino acid sequence set forth in SEQ ID NO:
• 1;
• (b) a polypeptide comprising an amino acid sequence having at least 70% (e.g., at least 95%, at least 96%, at least 97%, at least 98% or at least 99%) identity to the amino acid sequence set forth in SEQ ID NO:
• 1, but less than 100% sequence identity with the amino acid sequence set forth in SEQ ID NOs:
• 2 or 3, wherein said polypeptide is capable of modifying the C7-atom of deoxynivalenol (DON) and/or DON derivative selected from the group consisting of:
• 3-keto Deoxynivalenol, 3-epi Deoxynivalenol, 15-Acetyldeoxynivalenol, Nivalenol, Fusarenon-X (4-acetylnivalenol), 3-amino Deoxynivalenol;
• (ii) a nutritive source and/or a substrate for at least one of the polypeptides (a)-(b), preferably said nutritive source and/or substrate comprising DON and/or said DON derivative/s;
• preferably said polypeptide is a recombinant and/or isolated polypeptide;
• further preferably said altering toxicity comprising one or more of the following:
• detoxifying and/or intramolecularly rearranging DON and/or DON derivative/s;
• most preferably said polypeptide is capable of modifying the C7-atom of DON and/or DON derivative/s.

A method for modifying the C7-atom of deoxynivalenol (DON) and/or DON derivative selected from the group consisting of:

• 3-keto Deoxynivalenol, 3-epi Deoxynivalenol, 15-Acetyldeoxynivalenol, Nivalenol, Fusarenon-X (4-acetylnivalenol), 3-amino Deoxynivalenol, said method comprising:
• i) providing one or more of the following polypeptides:
• (a) a polypeptide comprising the amino acid sequence set forth in SEQ ID NO:
• 1;
• (b) a polypeptide comprising an amino acid sequence having at least 70% (e.g., at least 95%, at least 96%, at least 97%, at least 98% or at least 99%) identity to the amino acid sequence set forth in SEQ ID NO:
• 1, but less than 100% sequence identity with the amino acid sequence set forth in SEQ ID NOs:
• 2 or 3, wherein said polypeptide is capable of modifying the C7-atom of deoxynivalenol (DON) and/or DON derivative selected from the group consisting of:
• 3-keto Deoxynivalenol, 3-epi Deoxynivalenol, 15-Acetyldeoxynivalenol, Nivalenol, Fusarenon-X (4-acetylnivalenol), 3-amino Deoxynivalenol;
• ii) applying one or more of (a)-(b) to DON and/or said DON derivative/s;
• preferably said one or more polypeptides are one or more recombinant and/or isolated polypeptides.

Use of one or more of the following:

• (a) a polypeptide comprising the amino acid sequence set forth in SEQ ID NO:
• 1;
• (b) a polypeptide comprising an amino acid sequence having at least 70% (e.g., at least 95%, at least 96%, at least 97%, at least 98% or at least 99%) identity to the amino acid sequence set forth in SEQ ID NO:
• 1, but less than 100% sequence identity with the amino acid sequence set forth in SEQ ID NOs:
• 2 or 3, wherein said polypeptide is capable of modifying the C7-atom of deoxynivalenol (DON) and/or DON derivative selected from the group consisting of:
• 3-keto Deoxynivalenol, 3-epi Deoxynivalenol, 15-Acetyldeoxpivalenol, Nivalenol, Fusarenon-X (4-acetylnivalenol), 3-amino Deoxynivalenol;
• (c) composition, kit, foodstuff, intermediate foodstuff;
• fodder, intermediate fodder;
• feed, intermediate feed;
• additive, intermediate additive;
• detoxifying agent, intermediate detoxifying agent, nutritional supplement, intermediate nutritional supplement, prebiotic, intermediate prebiotic or mixture/s thereof according to any one of the preceding claims;
• for/in one or more of the following:
• i) modifying the C7-atom of DON and/or DON derivative/s;
• ii) producing a foodstuff, intermediate foodstuff;
• fodder, intermediate fodder;
• feed, intermediate feed;
• additive, intermediate additive;
• detoxifying agent, intermediate detoxifying agent;
• nutritional supplement, intermediate nutritional supplement;
• prebiotic, intermediate prebiotic and/or mixture/s thereof;
• iii) degrading DON and/or said DON derivative/s and/or altering toxicity of DON and/or said DON derivative/s, preferably said one or more polypeptides are recombinant and/or isolated polypeptides;
• further preferably said altering toxicity comprising one or more of the following:
• detoxifying and/or intramolecularly rearranging DON and/or said DON derivative/s;
• most preferably said one or more polypeptides are capable of modifying the C7-atom of DON and/or said DON derivative/s;
• iv) any combination of (i)-(iii);
• v) use according to any one (i)-(iv), wherein said use is an in vitro, ex vivo or in vivo use.