US 2023/0399667 A1Published application

Polynucleotide Having Promoter Activity and Application of Polynucleotide in Producing Amino Acid

AssigneeTianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences

InventorsPing Zheng, Jiao Liu, Jibin Sun, Wenjuan Zhou, Tuo Shi, Xuan Guo, Yanhe Ma

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Abstract

Provided are a polynucleotide having promoter activity and an application of the polynucleotide in producing an amino acid. Also disclosed are a transcription expression cassette, a recombinant expression vector, and a recombinant host cell which contain the polynucleotide, and a method for enhancing expression of a target gene, a method for preparing a protein, and a method for producing an amino acid. The polynucleotide having the promoter activity is a mutant of a polynucleotide having the sequence as shown in SEQ ID NO: 9. Compared with the polynucleotide having the sequence as shown in SEQ ID NO: 9, the promoter activity of the mutant is significantly enhanced, thereby promoting the stable and efficient expression of the target gene.

Claims20

Dependent claims

A polynucleotide having promoter activity, wherein the polynucleotide is selected from the group shown in any one of the following (i)-(ii):

(i) a mutant of a polynucleotide having the sequence as set forth in SEQ ID NO:
9, comprising a mutated nucleotide at one or more positions in position 292 to position 300 of the sequence as set forth in SEQ ID NO:
9;
(ii) a polynucleotide having at least 90%, optionally at least 95%, preferably at least 97%, more preferably at least 98%, and most preferably at least 99% sequence identity compared with the sequence as set forth in (i) and does not comprise the sequence as set forth in SEQ ID NO:
9;
wherein the mutant has higher promoter activity than that of the polynucleotide having the sequence as set forth in SEQ ID NO:
9;
and
the nucleotide sequence of the mutant at position 292 to position 300 of the sequence as set forth in SEQ ID NO:
9 is not selected from ATGCATTGT.

7 more elements

Dependencies are inferred from the claim text.