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US 2023/0399658 A1Published application
Aav chimeras
AssigneeUniversity of Florida Research Foundation, Inc.
InventorsMavis Agbandje-McKenna, Mario Mietzsch, Robert McKenna
Life of this patent
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Abstract
Provided herein are compositions and methods for packaging a recombinant adeno-associated virus (rAAV) particle comprising using inverted terminal repeats (ITRs) and rep genes of different serotypes and/or using chimeric rep genes.
Claims18
1-98. (canceled)
A method for producing a recombinant adeno-associated virus (AAV) particle that comprises a gene of interest in a cell, the method comprising:
- contacting the cell with:
- (a) a first nucleic acid sequence comprising a rep gene that encodes a chimeric rep protein, wherein the rep gene comprises at least one nucleic acid sequence from a rep gene of a first AAV serotype and at least one nucleic acid sequence from a rep gene of a second AAV serotype, wherein the first AAV serotype and the second AAV serotype are different;
- and
- (b) a second nucleic acid sequence that comprises a gene of interest and an inverted terminal repeat (ITR),
- thereby producing the recombinant AAV particle comprising the gene of interest.
2 more elements
Dependencies are inferred from the claim text.