Increasing levels of nicotinic alkaloids in plants
Assignee22nd Century Limited, LLC
InventorsTakashi HASHIMOTO, Masataka KAJIKAWA
Life of this patent
Estimating the term…
Abstract
Four genes, A622, NBB1, PMT, and QPT, can be influenced for increasing nicotinic alkaloid levels in Nicotiana plants, as well as for synthesizing nicotinic alkaloids in non-nicotine producing plants and cells. In particular, overexpressing one or more of A622, NBB1, PMT, and QPT may be used to increase nicotine and nicotinic alkaloid levels in tobacco plants. Non-nicotine producing cells can be engineered to produce nicotine and related compounds by overexpressing A622 and NBB1.
Claims21
1-26. (canceled)
A cell modified to express a A662 enzyme and a NBB1 enzyme, the cell comprising an isolated nucleic molecule encoding A662 and NBB1, wherein:
- (a) the isolated nucleic acid molecule encoding A662 comprises at least one of:
- (i) the nucleotide sequence set forth in SEQ ID NO:
- 3;
- and
- (ii) a nucleotide sequence that encodes a polypeptide having the amino acid sequence set forth in SEQ ID NO:
- 4, and
- (b) the isolated nucleic acid molecule encoding NBB1 comprises at least one of:
- (i) the nucleotide sequence set forth in SEQ ID NO:
- 1;
- and
- (ii) a nucleotide sequence that encodes a polypeptide having the amino acid sequence set forth in SEQ ID NO:
- 2.
A cell modified to express a NBB1 enzyme, the cell comprising an isolated nucleic molecule encoding NBB1, wherein the isolated nucleic acid molecule encoding NBB1 comprises at least one of:
- (a) the nucleotide sequence set forth in SEQ ID NO:
- 1;
- and
- (b) a nucleotide sequence that encodes a polypeptide having the amino acid sequence set forth in SEQ ID NO:
- 2.
A cell modified to express a A662 enzyme and a NBB1 enzyme, the cell comprising an isolated nucleic molecule encoding A662 and an isolated nucleic molecule encoding NBB1, wherein:
- (a) the isolated nucleic acid molecule encoding A662 comprises at least one of:
- (i) the nucleotide sequence set forth in SEQ ID NO:
- 3;
- and
- (ii) a nucleotide sequence that encodes a polypeptide having the amino acid sequence set forth in SEQ ID NO:
- 4, and
- (b) the isolated nucleic acid molecule encoding NBB1 comprises at least one of:
- (i) the nucleotide sequence set forth in SEQ ID NO:
- 1;
- and
- (ii) a nucleotide sequence that encodes a polypeptide having the amino acid sequence set forth in SEQ ID NO:
- 2.
Dependencies are inferred from the claim text.