Plasmid stabilisation
AssigneeOxford University Innovation Limited
InventorsChristoph TANG, Gareth MCVICKER, Sarah HOLLINGSHEAD
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Abstract
The invention related to an isolated plasmid encoding a bacterial VapBC toxin-antitoxin system, wherein the VapBC toxin-antitoxin system comprises a modified VapB amino acid sequence arranged to increase the maintenance of the plasmid in a bacterial host, wherein the modification to VapB comprises or consists of a substitution of at least one of Q12, F6, F51 and F60 residues relative to Shigella sonnei wild-type VapB or Shigella flexneri wild-type VapB, or structurally equivalent residues of Shigella sonnei wild-type VapB or Shigella flexneri wild-type VapB in a homologue thereof; and associated methods, uses and host cells.
Claims27
An isolated plasmid encoding a bacterial VapBC toxin-antitoxin system, wherein the VapBC toxin-antitoxin system comprises a modified VapB amino acid sequence arranged to increase the maintenance of the plasmid in a bacterial host, wherein the modification to VapB comprises or consists of a substitution of at least one of Q12, F6, F51 or F60 residues relative to Shigella sonnei wild-type VapB or Shigella flexneri wild-type VapB, or structurally equivalent residues of Shigella sonnei wild-type VapB or Shigella flexneri wild-type VapB in a homologue thereof.
A nucleic acid encoding a modified VapB amino acid sequence arranged to increase the maintenance of a plasmid or genetic element in a bacterial host, wherein the modification to VapB comprises or consists of a substitution of at least one of Q12, F6, F51 and F60 residues relative to Shigella sonnei wild-type VapB or Shigella flexneri wild-type VapB, or structurally equivalent residues of Shigella sonnei wild-type VapB or Shigella flexneri wild-type VapB in a homologue thereof.
A modified VapB polypeptide, the modification comprising or consisting of a substitution of at least one of Q12, F6, F51 or F60 residues relative to Shigella sonnei wild-type VapB, or structurally equivalent residues of Shigella sonnei wild-type VapB in a homologue thereof.
23-24. (canceled)
Dependencies are inferred from the claim text.