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US 2023/0399631 A1Published application
Rna-guided endonuclease fusion polypeptides and methods of use thereof
AssigneeThe Regents of the University of California
InventorSchaked Omer Halperin
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Abstract
The present disclosure provides a fusion polypeptide comprising: a) an enzymatically active RNA-guided endonuclease that introduces a single-stranded break in a target DNA; and b) an error-prone DNA polymerase. The present disclosure provides a system comprising: a) a fusion polypeptide of the present disclosure; and b) a guide RNA. The present disclosure provides a cell comprising a fusion polypeptide of the present disclosure, or a system of the present disclosure. The present disclosure provides a method of mutagenizing a target polynucleotide.
Claims25
1-84. (canceled)
An editing system comprising:
- a) an enzymatically active RNA-guided endonuclease that introduces a break in at least one strand in a target DNA;
- b) a DNA polymerase that binds to the target DNA 5′ to the break;
- and
- c) a guide RNA that binds the endonuclease;
- d) wherein the endonuclease, DNA polymerase, and guide RNA are exogenously introduced into a cell, or are encoded by one or more exogenously introduced nucleic acids.
2 more elements
Dependencies are inferred from the claim text.