Methods, compositions, and kits for expanding natural killer cells
AssigneeRegents of the University of Minnesota
InventorsBranden Scott Moriarity, Jae Woong Chang, Beau R. Webber
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Abstract
Provided herein are methods, compositions, and kits for expanding natural killer cells in the absence of feeder cells.
Claims234
A method of expanding an NK cell comprising:
- contacting an NK cell disposed in a liquid culture medium, in the absence of a feeder cell, with (i) a first polypeptide comprising B7-H6 or a functional fragment thereof, or an anti-NKp30 antigen-binding domain, (ii) a second polypeptide comprising 4-1BBL or a functional fragment thereof, and (iii) a third polypeptide comprising ICAM-1 or a functional fragment thereof, under conditions sufficient for expansion of the NK cell.
The method of claim 22, wherein two or more of the first polypeptide, the second polypeptide, and the third polypeptide are directly attached to the one or more substrate(s).
The method of claim 55, wherein the liquid culture medium comprises about U/mL to about 200 U/mL of IL-2 or a functional fragment thereof.
A method of expanding an NK cell comprising:
- contacting an NK cell disposed in a liquid culture medium, in the absence of a feeder cell, with (i) a first polypeptide comprising B7-H6 or a functional fragment thereof, or an anti-NKp30 antigen-binding domain, (ii) a second polypeptide comprising 4-1BBL or a functional fragment thereof, (iii) a third polypeptide comprising ICAM-1 or a functional fragment thereof, and (iv) a fourth polypeptide comprising IL-21 or a functional fragment thereof, under conditions sufficient for expansion of the NK cell.
The method of any one of claims 100-107, wherein one or more of the first polypeptide, the second polypeptide, the third polypeptide, and the fourth polypeptide further comprises a linker.
The method of any one of claims 150-154, wherein the replenishing of the liquid culture medium occurs about every one or two days.
A kit comprising:
- one or more substrate(s) that together comprise:
- (i) a first polypeptide comprising B7-H6 or a functional fragment thereof, or an anti-NKp30 antigen-binding domain, (ii) a second polypeptide comprising 4-1BBL or a functional fragment thereof, and (iii) a third polypeptide comprising ICAM-1 or a functional fragment thereof, directly or indirectly attached to the one or more substrate(s).
A kit comprising:
- one or more substrate(s) that together comprise:
- (i) a first polypeptide comprising B7-H6 or a functional fragment thereof, or an anti-NKp30 antigen-binding domain, (ii) a second polypeptide comprising 4-1BBL or a functional fragment thereof, (iii) a third polypeptide comprising ICAM-1 or a functional fragment thereof, and (iv) a fourth polypeptide comprising IL-21 or a functional fragment thereof, directly or indirectly attached to the one or more substrate(s).
Dependencies are inferred from the claim text.