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US 2023/0399598 A1Published application
Vertical thermal gradient equipment and method for progressive sperm sorting
AssigneeIpreg Incorporation.
InventorsChing-Wen CHANG, Chen-Yen CHUNG, Chung-Hsien HUANG, Bor-Ran LI
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Abstract
This disclosure relates to an apparatus and method for sorting motile sperms from crude semen. the enrichment of a population of spermatozoa with capacitated spermatozoa can be achieved by providing a 1-to-4-degree vertical temperature difference in combination with the swim-up method.
Claims14
An apparatus for processing sperm, comprising:
- an upper chamber and a bottom chamber arranged vertically;
- a collection port connected to the upper chamber;
- an injection port connected to the bottom chamber;
- a porous layer that placed at the interface between the upper and bottom chamber to allow particles communication between them and delay thermal equilibrium therebetween;
- and
- a temperature control unit means for maintaining the temperature of the upper chamber higher than that of the bottom chamber.
3 more elements
Dependencies are inferred from the claim text.