Novel anti-cd47 antibodies and uses thereof
OwnerZhengyi Wang, Wei Cao +2inventors
InventorsZhengyi Wang, Wei Cao, Bingshi Guo, Cong Xu
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Abstract
Provided herein are CD47 antibodies and immunologically active fragments thereof that have low immunogenicity in humans and cause low or no level of red blood cell depletion or hemagglutination. Also provided are as well as pharmaceutical compositions containing such antibodies or antibody fragments, as well as methods of treatment using such antibodies, e.g., as single agents or in combination with other therapeutic agent (s).
Claims64
An antibody or immunologically active fragment thereof that specifically binds to human CD47 (hCD47), comprising:
- (a) a heavy chain variable (VH) domain that comprises (1) a glutamic acid residue (E) at its N-terminus;
- (2) a CDR-H1 comprising RAWMN (SEQ ID NO:
- 5);
- (3) a CDR-H2 comprising RIKRKTDGETTDYAAPVKG (SEQ ID NO:
- 6);
- (4) a CDR-H3 comprising SNRAFDI (SEQ ID NO:
- 7);
- and (5) a serine (S) at its C-terminus;
- and
- (b) a light chain variable (VL) domain that comprises (1) a CDR-L1 comprising KSSQSVLYAGNNRNYLA (SEQ ID NO:
- 8);
- (2) a CDR-L2 comprising QASTRAS (SEQ ID NO:
- 9);
- and (3) a CDR-L3 comprising QQYYTPPLA (SEQ ID NO:
- 10).
A method of treating cancer in a subject, comprising administering an effective amount of an anti-CD47 antibody to the subject,
- wherein the anti-CD47 antibody comprises
- (a) a heavy chain variable domain (VH) that comprises (1) a CDR-H1 comprising RAWMN (SEQ ID NO:
- 5);
- (2) a CDR-H2 comprising RIKRKTDGETTDYAAPVKG (SEQ ID NO:
- 6);
- and (3) a CDR-H3 comprising SNRAFDI (SEQ ID NO:
- 7);
- (b) a light chain variable (VL) domain that comprises (1) a CDR-L1 comprising KSSQSVLYAGNNRNYLA (SEQ ID NO:
- 8);
- (2) a CDR-L2 comprising QASTRAS (SEQ ID NO:
- 9);
- and (3) a CDR-L3 comprising QQYYTPPLA (SEQ ID NO:
- 10).
The method of claim 42 or 43, wherein the solid tumor is a lung tumor, an ovarian tumor, a colorectal tumor, a pancreatic tumor, a sarcoma tumor, a head and neck tumor, a gastric tumor, a renal tumor, or a skin tumor.
Dependencies are inferred from the claim text.