Anti-il5 nanoantibody and use thereof

Abstract

An anti-IL5 nanoantibody, and a coding sequence for coding the anti-IL5 nanoantibody, a corresponding expression vector, a host cell capable of expressing the anti-IL5 nanoantibody, and a method for producing the anti-IL5 nanoantibody are provided. The nanoantibody can specifically recognize IL5 from humans and cynomolgus monkeys, does not recognize IL5 from mice, and has a good binding activity. The nanoantibody has a good IL5/IL5R blocking activity. The blocking activity is obviously superior to that of a control antibody Nucala. The nanoantibody can effectively inhibit the proliferation of TF-1 cells induced by IL5, and the inhibition activity thereof is superior to that of the control antibody Nucala. The expression yield of the nanoantibody in Pichia pastoris can reach 13 g/L, and the purity of target protein expressed in the supernatant is high.

Dependencies are inferred from the claim text.