Back to results
US 2023/0399379 A1Published application
Expression of collagen peptide components in prokaryotic systems
AssigneesGelita AG, UniversitäT Bielefeld
InventorsSeverin Wedde, Joe Max Risse, Karl Friehs, Martin Hahn, Benjamin Ludwig
Life of this patent
Estimating the term…
Abstract
The present invention relates to methods for preparing hydroxylated collagen peptide components and to hydroxylated collagen peptide components, in particular hydroxylated collagen peptides, obtained from these methods.
Claims17
A method for preparing a recombinant hydroxylated collagen peptide component in prokaryotic systems comprising the steps of:
- (a) providing a prokaryotic expression system comprising at least one nucleotide sequence encoding at least one recombinant collagen peptide component and at least one nucleotide sequence encoding at least one prolyl 4-hydroxylase, wherein the nucleotide sequence encoding at least one recombinant collagen peptide component comprises the nucleotide sequence of at least one collagen peptide,
- b) cultivating the prokaryotic expression system in a culture medium under conditions which allow expression of the at least one recombinant collagen peptide component and the at least one prolyl 4-hydroxylase to obtain at least one hydroxylated collagen peptide component, wherein the collagen peptide component has a reduced hydrophobicity and/or a reduced proline content relative to the at least one collagen peptide encoded by the collagen peptide component encoding nucleotide sequence,
- c) recovering the hydroxylated collagen peptide component,
- wherein the at least one collagen peptide has the amino acid sequence motif (Gly-X-Y)n and at least 55% of the hydroxylations in the at least one collagen peptide are at a proline in the Y-position.
1 more element
A method for preparing a recombinant hydroxylated collagen peptide component in prokaryotic systems comprising the steps of:
- i) providing a prokaryotic expression system comprising at least one nucleotide sequence encoding at least one recombinant collagen peptide component and at least one nucleotide sequence encoding at least one proline 4-hydroxylase (PIN4H), wherein the nucleotide sequence encoding at least one recombinant collagen peptide component comprises the nucleotide sequence of at least one collagen peptide,
- ii) cultivating the prokaryotic expression system in a culture medium under conditions which allow expression of the at least one recombinant collagen peptide component and the at least one proline 4-hydroxylase (PIN4H) to obtain at least one hydroxylated collagen peptide component, wherein the collagen peptide component has a reduced hydrophobicity and/or a reduced proline content relative to the at least one collagen peptide encoded by the collagen peptide component encoding nucleotide sequence,
- iii) recovering the hydroxylated collagen peptide component.
Dependencies are inferred from the claim text.