Methods of treating glioblastoma

A method of treating unifocal glioblastoma in a subject in need thereof comprising

• a. intratumorally injecting into the subject an adenoviral vector, wherein the vector comprises:
• i. a first polynucleotide encoding an IL-12 p40 polypeptide comprising an amino acid sequence at least 85% identical to wild-type human IL-12 p40 polypeptide;
• ii. a second polynucleotide encoding an IL-12 p35 polypeptide comprising an amino acid sequence at least 85% identical to wild-type human IL-12 p35 polypeptide;
• iii. a third polynucleotide encoding a VP-16 transactivation domain-retinoic acid-X-receptor fusion protein (VP-16-RXR);
• and
• iv. a fourth polynucleotide encoding a Gal4 DNA binding domain and an ecdysone receptor (EcR) binding domain fusion protein (Gal4-EcR), wherein the VP-16-RXR fusion protein and the Gal4-EcR fusion protein form a ligand dependent transcription factor complex;
• and
• b. orally administering to the subject a diacylhydrazine ligand that activates the ligand-dependent transcription factor complex,
• thereby treating the unifocal glioblastoma in the subject.