Baculovirus expression vector
AssigneeIntervet Inc.
InventorAmaya Serrano Garcia
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Abstract
The invention concerns a baculovirus expression vector for recombinantly expressing an FMDV capsid precursor protein under control of a promoter, the expression vector comprising a nucleic acid sequence encoding the FMDV capsid precursor protein and the translational enhancers Syn2 land p10UTR. The invention further relates to a host cell comprising the baculovirus expression vector, a method of producing FMDV virus-like particles (VLPs), and a method of producing a vaccine.
Claims16
A baculovirus expression vector capable of recombinantly expressing a Foot and mouth disease virus (FMDV) capsid precursor protein under control of a promoter, the expression vector comprising:
- (i) a nucleic acid sequence encoding the FMDV capsid precursor protein,
- (ii) a translational enhancer Syn21 located within the 5′ untranslated region (UTR) of the nucleic acid sequence (i) encoding the FMDV capsid precursor protein, and
- (iii) a translational enhancer P10UTR, located within the 3′UTR of the nucleic acid sequence (i) encoding the FMDV capsid precursor protein.
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Dependencies are inferred from the claim text.