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US 2023/0398545 A1Published application
Assay using sample thickness multiplexing
AssigneeEssenlix Corporation
InventorsStephen Y. CHOU, Wei DING, Ji Qi, Yufan Zhang
Life of this patent
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Abstract
One aspect of the present invention is to provide the device and methods for performing an assay that uses the multiplexing of sample thicknesses on the same plate. The sample thickness multiplexing can offer many information that is unavailable in using a single sample thickness.
Claims8
A device for analyzing an analyte in a sample, comprising:
- a first plate, a second plate, spacers, and at least one imager, wherein:
- i. the first and second plates face each other;
- ii. the first plate has, on its inner surface, a first and a second sample contact areas at different locations, and the second plate has, on its inner surface, a first and a second sample contact area at different locations that correspond to and face the first and the second sample contact areas of the first plate, respectively, wherein the sample contact areas are the areas for contacting a sample that contains or is suspected to contain an analyte;
- iii. the spacers are between the first plate and the second plate;
- iv. the spacers and the surface of the sample contact areas are configured to make a first spacing height and a second spacing height different from each other, wherein the first spacing height is the spacing between the first sample contact surface on the first plate and its corresponding sample contact area on the second plate, and the second spacing height is the spacing between the second sample contact surface on the first plate and its corresponding sample contact are on the second plate, wherein the first and second spacing heights are 250 um or less;
- and
- v. the at least one imager is configured to image the samples in the first and second sample contact area, and to measure an optical signal related to the analyte in the first and second spacing height.
4 more elements
A device for analyzing an analyte in a whole blood sample, comprising:
- a first plate, a second plate, spacers, and at least one imager, wherein:
- i. the first and second plates face each other;
- ii. the first plate has, on its inner surface, a first and a second sample contact areas at different locations, and the second plate has, on its inner surface, a first and a second sample contact area at different locations that is a correspond to and face the first and the second sample contact areas of the first plate, respectively, wherein the sample contact areas are the areas for contacting a whole blood sample that contains or is suspected to contain an analyte;
- iii. the spacers are between the first plate and the second plate;
- iv. the spacers and the surface of the sample contact areas are configured to make a first spacing height and a second spacing height different from each other, wherein the first spacing height is the spacing between the first sample contact surface on the first plate and its corresponding sample contact area on the second plate, and the second spacing height is the spacing between the second sample contact surface on the first plate and its corresponding sample contact are on the second plate, wherein the first and second spacing heights are 250 um or less;
- and
- v. the at least one imager is configured to image the samples in the first and second sample contact area, and to measure an optical signal related to the analyte in the first and second spacing height;
- wherein the sample is whole blood, and the analyte comprises red blood cells, white blood cells, platelets, and hemoglobin, wherein the first spacing height is a single value selected from a range of 3.5 um to 6.5 um, and the second spacing height is a single value selected from a range of 10 um to 120 um, and wherein the first spacing height is used to measure the red blood cell and the platelets, and the second spacing height is used to measure the white blood cells and hemoglobin.
5 more elements
Dependencies are inferred from the claim text.