Composition comprising polyethylene glycol conjugated to indocyanine green and methods of use
AssigneeBoard of Regents, The University of Texas System
InventorsJie ZHENG, Bujie DU, Mengxiao YU
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Abstract
The present disclosure provides polyethylene glycol-indocyanine green conjugates as well as the methods of using such conjugates, such as diagnosis, tissue imaging, temporal monitoring, and treatment.
Claims34
A method of diagnosing a disease or condition associated with abnormal expression of an influx or efflux transporter in a subject, comprising:
- administering to the subject a composition comprising polyethylene glycol (PEG) conjugated to indocyanine green (ICG) (“ICG-PEG conjugate”);
- determining a concentration of the ICG-PEG conjugate in a biological sample obtained from the subject;
- comparing the concentration of the ICG-PEG conjugate with a reference level;
- and
- determining that the subject has the disease or condition if the concentration of the ICG-PEG conjugate is significantly greater or lower than the reference level.
A method of diagnosing a disease or condition associated with abnormal expression of an influx or efflux transporter in a subject, comprising:
- administering to the subject a composition comprising polyethylene glycol (PEG) conjugated to indocyanine green (ICG) (“ICG-PEG conjugate”);
- measuring an intensity of a signal from the ICG-PEG conjugate in a tissue of the subject;
- comparing the intensity with a reference level;
- and
- determining that the subject has the disease or condition if the intensity is significantly greater or lower than the reference level.
A method of monitoring kidney secretion function of a subject, comprising:
- administering to the subject a composition comprising polyethylene glycol (PEG) conjugated to indocyanine green (ICG) (“ICG-PEG conjugate”);
- determining a first concentration of the ICG-PEG conjugate in a first biological sample obtained from the subject at a first time point;
- determining a second concentration of the ICG-PEG conjugate in a second biological sample obtained from the subject at a second time point, wherein the second time point is after the first time point;
- determining renal clearance kinetics based on the first concentration and the second concentration;
- and
- optionally comparing the renal clearance kinetics with a reference level.
A method of monitoring kidney secretion function of a subject, comprising:
- administering to the subject a composition comprising polyethylene glycol (PEG) conjugated to indocyanine green (ICG) (“ICG-PEG conjugate”);
- measuring, at a first time point, a first intensity of a signal from the ICG-PEG conjugate in a tissue of the subject;
- and
- measuring, at a second time point, a second intensity of a signal from the ICG-PEG conjugate in the tissue of the subject.
A method of treating a disease or condition associated with abnormal expression of an influx or efflux transporter in a subject in need thereof, comprising administering to the subject a composition comprising polyethylene glycol (PEG) conjugated to indocyanine green (ICG) (“ICG-PEG conjugate”).
A method of detecting a liver disease in a subject, comprising:
- administering to the subject a composition comprising polyethylene glycol (PEG) conjugated to indocyanine green (ICG) (“ICG-PEG conjugate”), wherein the PEG has a molecular weight of at least 100 Da to less than 2 kDa;
- determining a concentration of the ICG-PEG conjugate in a urine sample obtained from the subject;
- comparing the concentration of the ICG-PEG conjugate with a reference level;
- and
- determining that the subject has the liver disease when the concentration of the ICG-PEG conjugate is significantly less than the reference level.
A method of measuring an expression level of an influx or efflux transporter in a subject, comprising:
- administering to the subject a composition comprising polyethylene glycol (PEG) conjugated to indocyanine green (ICG) (“ICG-PEG conjugate”);
- determining a concentration of the ICG-PEG conjugate in a biological sample obtained from the subject;
- and
- determining the expression level of the influx or efflux transporter based on the concentration of the ICG-PEG conjugate.
A method of measuring an expression level of an influx or efflux transporter in a subject, comprising:
- administering to the subject a composition comprising polyethylene glycol (PEG) conjugated to indocyanine green (ICG) (“ICG-PEG conjugate”);
- measuring an intensity of a signal from the ICG-PEG conjugate in a tissue of the subject;
- and
- determining the expression level of the influx or efflux transporter based on the intensity.
A composition comprising indocyanine (ICG), polyethylene glycol (PEG), and a secondary moiety, wherein ICG and the secondary moiety are each independently conjugated to PEG.
Dependencies are inferred from the claim text.