Microfluidic determination of heterogeneous objects
AssigneeAmberstone Biosciences, Inc.
InventorsGeorge WU, Per Niklas HEDDE, Xiaoming CHEN
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Abstract
Provided are microfluidic systems and methods for detecting and sorting of droplets comprising heterogeneous particulate objects such as single cells and non-cell particles, including a variety of eukaryotic and bacterial cells, for a variety of bioassay applications. The systems and methods described herein, when implemented in whole or in part, will make relevant microfluidic based tools available for a variety of applications in biotechnology including antibody discovery, immuno-therapeutic discovery, high-throughput single cell analysis, target-specific compound screening, and synthetic biology screening.
Claims21
1-82. (canceled)
A system for detecting one or more objects in a droplet, the system comprising:
- a) a microfluidic device comprising a first channel comprising a plurality of water-in-oil droplets, a droplet of the plurality of droplets comprising one or more objects therein;
- b) a first optical detector corresponding to a first point of detection disposed along the first channel;
- and
- c) a first optical element configured to provide dual focusing along the first channel at the first point of detection;
- wherein the dual focusing is facilitated by a first beam at a first focus and a second beam at a second focus, wherein the first focus and the second focus are on axially separate focal volumes.
Dependencies are inferred from the claim text.