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US 2023/0390771 A1Published application
Method for concentrating droplets in an emulsion
Assignee10x Genomics, Inc.
InventorsIvan AKHREMICHEV, Lynna CHEN, Martin SAUZADE
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Abstract
The invention provides devices for forming droplets and concentrating droplets, and methods of their use. During operation of the devices, droplets are generated using two liquid phases. Once droplet formation is complete excess continuous phase is removed by the use of one or more pressure differentials.
Claims21
A method of concentrating droplets in an emulsion comprising:
- a) providing a device comprising:
- i) a first channel having a first proximal end, a first distal end, a first depth, and a first width;
- ii) a droplet source region in fluid communication with the first distal end of the first channel, wherein the droplet source region has a width or depth greater than the first width or first depth;
- and
- iii) a collection reservoir in fluid communication with the droplet source region that collects droplets formed in the droplet source region;
- b) flowing a first liquid from the first proximal end to the droplet source region to produce an emulsion of droplets of the first liquid in a second liquid in the collection reservoir;
- and
- c) reducing the volume of the second liquid in the emulsion by applying a first pressure differential for a first period of time and a second pressure differential for a second period of time to produce a concentrated emulsion.
5 more elements
(canceled)
Dependencies are inferred from the claim text.