Device for detection of cellular stress
AssigneesTrustees of Boston University, Northwestern University
InventorsJi-Xin Cheng, Junjie Li, Yuying Tan, Daniela Matei, Guangyuan Zhao
Life of this patent
Estimating the term…
Abstract
Disclosed is an assay for determining resistance in a target cell or tissue to a therapy associated with cellular stress using chemical microscopy and high-throughput single cell analysis to determine functional metabolic alteration, including determining metabolic reprogramming in a target cell or tissue to a therapy associated with cellular stress, and methods of using the assays.
Claims18
An assay for determining resistance in a target cell to a therapy, the assay comprising:
- measuring glucose and glycolysis derived anabolism in the target cell;
- measuring fatty acid uptake and oxidation in the target cell;
- and
- determining a change from glucose anabolism in the target cell to fatty acid uptake and oxidation energy metabolism.
The assay of claim 1, wherein the therapy is a cancer therapy. (New) The assay of claim 9, wherein the cancer therapy is selected from chemotherapy, radiotherapy, immunotherapy, targeted therapy, hormone therapy, light therapy, laser therapy, photodynamic therapy, and combinations thereof.
The method of claim 13, wherein the cancer therapy is selected from chemotherapy, radiotherapy, immunotherapy, targeted therapy, hormone therapy, light therapy, laser therapy, photodynamic therapy, and combinations thereof. (New) The method of claim 14, wherein the cancer therapy is chemotherapy comprising one or more platinum-based agents, nitrosoureas, anti-metabolites, anti-tumor antibiotics, plant alkaloids, topoisomerase inhibitors, mitotic inhibitors, hormonal agents, corticosteroids, biological response modifiers, carboplatin, and/or oxaliplatin.
Dependencies are inferred from the claim text.