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US 2023/0382875 A1Published application
Compounds, compositions, and methods for isolation of nucleic acids
AssigneePromega Corporation
InventorsZhiyang Zeng, Wenhui Zhou, Douglas R. Storts, Poncho Meisenheimer, Ian Marozas, Andrew Taft, Spencer Hermanson, Connor Fitzgerald, Kasen Riemersma
Life of this patent
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Abstract
Provided herein are compounds, compositions, and methods for rapid isolation of nucleic acids from a sample.
Claims24
A composition comprising:
- a solid surface;
- a linker covalently attached to the solid surface;
- and
- a moiety of formula (I) covalently attached to the linker:
- wherein:
- X is selected from O, S, S(O), and S(O)2;
- R1, R2, R3, R4, R5, R6, R7, and R8 are each independently selected from hydrogen, C1-C6 alkyl, C2-C6 alkenyl, C2-C6 alkynyl, C1-C6 haloalkyl, C3-C6 cycloalkyl, aryl, heteroaryl, heterocyclyl, halo, cyano, nitro, —ORa, —SRb, —C(O)ORc, —C(O)NRdRe, —OC(O)NRfRg, —NRhC(O)NRiRj, and —NRhC(S)NRiRj, wherein each Ra, Rb, Rc, Rd, Re, Rf, Rg, Rh, Ri, and Rj is independently selected from hydrogen, C1-C6 alkyl, C2-C6 alkenyl, C2-C6 alkynyl, C1-C6 haloalkyl, C3-C6 cycloalkyl, and aryl.
4 more elements
(canceled)
(canceled)
-43. (canceled)
A compound of formula (IIa):
- or a salt thereof, wherein:
- X is selected from O, S, S(O), and S(O)2;
- L is a linker;
- each Ra is independently selected from hydroxy, C1-C6 alkoxy, and halo;
- and
- R1, R2, R3, R4, R5, R6, R7, and R8 are each independently selected from hydrogen, C1-C6 alkyl, C2-C6 alkenyl, C2-C6 alkynyl, C1-C6 haloalkyl, C3-C6 cycloalkyl, aryl, heteroaryl, heterocyclyl, halo, cyano, nitro, —ORa, —SRb, —C(O)ORc, —C(O)NRdRe, —OC(O)NRfRg, NRhC(O)NRiRj, and —NRhC(S)NRiRj, wherein each Ra, Rb, Rc, Rd, Re, Rf, Rg, Rh, Ri, and Rj is independently selected from hydrogen, C1-C6 alkyl, C2-C6 alkenyl, C2-C6 alkynyl, C1-C6 haloalkyl, C3-C6 cycloalkyl, and aryl.
3 more elements
-53. (canceled)
Dependencies are inferred from the claim text.