Nucleic acid crosslinking reagents and uses thereof
OwnerSubhanjan Mondal, Nathan Feirer +5inventors
InventorsSubhanjan Mondal, Nathan Feirer, Min Zhou, Michael Scurria, Wenhui Zhou, Said Goueli, James J. Cali
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Abstract
Provided herein multifunctional compounds that bind and modify nucleic acids, forming crosslinks between the nucleic acid strands. In particular embodiments, the multifunctional nucleic-acid binding/modifying compounds are selective for nucleic acids of non-viable cells and viruses (i.e., they are excluded from or degraded within viable cells and viruses), and therefore find use in methods for detecting or discriminating viable and non-viable cells/viruses and nucleic acids therefrom.
Claims31
A multifunctional compound or a salt thereof, the multifunctional compound comprising:
- (A) a RNA binding moiety (“RAB moiety”) of formula (I):
- or a tautomer or a salt thereof, wherein:
- R1 and R2 are each independently selected from hydrogen, C1-C6 alkyl, C2-C6 alkenyl, C2-C6 alkynyl, aryl, and aryl-C1-C6 alkyl, each of which is optionally substituted with 1-3 substituents, or R1 and R2 are taken together with the nitrogen atom to which they are attached to form an optionally substituted heterocyclyl or heteroaryl ring;
- and
- R3 is absent or is C1-C6 alkyl;
- (B) a live/dead cell differentiating moiety (“LDCD moiety”);
- and
- (C) a nucleic acid modifying moiety (“NAM moiety”) comprising a bischloroethylamine (nitrogen mustard) moiety, a platinum-based moiety, a 1-(chloromethyl)-2,3-dihydro-1H-benzo[e]indolyl moiety, or a pyrrolo[2,1-c][1,4]benzodiazepine (PBD) moiety.
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Dependencies are inferred from the claim text.