Targeted rna editing by leveraging endogenous adar using engineered rnas
AssigneePeking University
InventorsWensheng Wei, Zongyi Yi, Liang Qu, Feng Tian, Chunhui Wang, Shiyou Zhu, Zhuo Zhou
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Abstract
The present application provides methods for editing RNA by introducing a deaminase-recruiting RNA in a host cell for deamination of an adenosine in a target RNA. The present application further provides deaminase-recruiting RNAs used in the RNA editing methods and compositions and kits comprising the same.
Claims21
1-43. (canceled)
A method of modulating splicing in a target pre-messenger RNA comprising a target adenosine in a host cell, comprising introducing a deaminase-recruiting RNA (dRNA) or a construct comprising a nucleic acid encoding the dRNA into the host cell, wherein:
- (1) the dRNA comprises a targeting RNA sequence that is at least partially complementary to the target pre-messenger RNA;
- (2) the dRNA is a circular RNA or capable of forming a circular RNA within the host cell;
- and
- (3) the dRNA hybridizes with the target pre-messenger RNA and recruits ADAR to the target pre-messenger RNA, thereby modulating the splicing of the target pre-messenger RNA.
Dependencies are inferred from the claim text.