Esterase and methods of use, thereof
AssigneeDanisco US Inc.
InventorsFENG GUO, HELONG HAO, BRADLEY KELEMEN, AMY DEMING LIU, KEFENG NI, ZHEN QIAN, ZHONGMEI TANG
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Abstract
The present disclosure relates to a method and a composition comprising an esterase for enzymatic surface modification of a polyester. The present disclosure also relates to a method of degradation or hydrolysis of an insoluble plant material.
Claims20
A method for modifying an ester bond-containing polymer, comprising:
- contacting the ester bond-containing polymer with an effective amount of the polypeptide having polymer degradation or hydrolysis activity, wherein the polypeptide having at least about 80%, at least about 85%, at least about 90%, at least about 95%, at least about 99%, amino acid sequence identity, or identical to SEQ ID NO:
- 2.
The method of any of the preceding claims, further comprise contacting ester bond-containing polymer with one or more cleaning agents selected from the group consisting of surfactants, builders, bleaching agents, dye transfer inhibiting agents, chelating agents, dispersants, polysaccharides, softening agents, suds suppressors, carriers, enzymes, enzyme stabilizing systems, polyacids, soil removal agents, anti-redeposition agents, hydrotropes, opacifiers, antioxidants, bactericides, dyes, perfumes, and brighteners, or a combination thereof.
The method of any of the preceding claims, further comprise contacting the ester bond-containing polymer with one or more enzymes selected from the group consisting of protease, hemicellulase, cellulase, peroxidase, esterase, xylanase, lipase, phospholipase, esterase, perhydrolase, cutinase, pectinase, pectate lyase, mannanase, keratinase, reductase, oxidase, phenoloxidase, lipoxygenase, ligninase, alpha-amylase, glucoamylase, pullulanase, beta-amylase, phytase, tannase, pentosanase, beta-glucanase, arabinosidase, laccase, transferrase, and a combination thereof.
A recombinant construct comprising a nucleotide sequence encoding a polypeptide having polymer degradation or hydrolysis activity, wherein said coding nucleotide sequence is operably linked to at least one regulatory sequence functional in a production host and is selected from the group consisting of:
- the nucleotide sequence set forth in SEQ ID NO:
- 2 and a nucleotide sequence with at least 80% sequence identity thereto, wherein said regulatory sequence is heterologous to the coding nucleotide sequence, or said regulatory sequence and coding sequence are not arranged as found together in nature.
A recombinant host cell comprising a nucleotide sequence encoding a polypeptide having polymer degradation or hydrolysis activity, wherein said coding nucleotide sequence is operably linked to at least one regulatory sequence functional in a production host and is selected from the group consisting of:
- the nucleotide sequence set forth in SEQ ID NO:
- 2 and a nucleotide sequence with at least 80% sequence identity thereto, wherein said regulatory sequence is heterologous to the coding nucleotide sequence, or said regulatory sequence and coding sequence are not arranged as found together in nature.
A method of degrading a plastic product containing at least one ester bond-containing polymer comprising contacting the plastic product with an effective amount of the polypeptide having polymer degradation or hydrolysis activity, wherein the polypeptide having at least about 80%, at least about 85%, at least about 90%, at least about 95%, at least about 99%, amino acid sequence identity, or identical to SEQ ID NO:
- 2.
Dependencies are inferred from the claim text.