Recombinant viruses, surface-engineered delivery systems and related methods
AssigneeDevacell, Inc.
InventorInanc ORTAC
Life of this patent
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Abstract
Provided herein are recombinant viruses and artificially coated delivery systems, and methods of use.
Claims76
A replication-competent oncolytic virus comprising:
- a coding-region for an essential-agent-depletion-enzyme;
- and
- a viral-genome deletion.
A method of engineering a tumor microenvironment of a patient in need thereof, said method comprising administering a combination of a first and second recombinant viruses to the patient,
- wherein the first recombinant virus is an oncolytic virus comprising a coding-region for an essential-agent-depletion-enzyme and a cancer-selective deletion;
- and
- wherein the second recombinant virus comprises at least one of:
- a coding-region for a prodrug-converting-enzyme that converts a prodrug into an active chemotherapeutic agent;
- or
- a coding-region for an essential-agent-depletion-enzyme.
A method of vaccinating an individual or generating an immune response against one or more target antigens in an individual in need thereof, comprising administering to the individual a surface-engineered recombinant virus that is a vaccine;
- thereby vaccinating the individual or generating an immune response against the one or more target antigens.
A method of administering a gene therapy to an individual in need thereof, comprising administering to the individual a surface-engineered recombinant gene-therapy virus comprising a transgene encoding a therapeutic protein;
- thereby administering the gene therapy to the individual.
A surface-engineered recombinant virus vaccine, said virus comprising:
- a recombinant Ad5 virus having a recombinant genome encoding SARS-CoV-2 spike protein, wherein at least a functional portion of E1 and E3 genes are deleted, or wherein the virus does not contain any native Ad5 viral genes;
- and
- an artificial coating layer encapsulating the recombinant Ad5 virus, wherein said coating layer comprises an effective amount of folate to bind a folate receptor on a cell, and wherein the artificial coating is selected from the group consisting of:
- silica, titanium oxide and calcium phosphate.
A method of re-engineering the surface of a virus having a native-envelope, said method comprising:
- removing the native-envelope from the virus to isolate a previously-enveloped-capsid;
- applying an artificial coating to the previously-enveloped-capsid.
A surface-re-engineered virus comprising:
- a previously-enveloped-capsid from a naturally occurring enveloped- virus;
- and
- an artificial coating layer surrounding the previously-enveloped- capsid.
A method of re-engineering a virus having a native-envelope, said method comprising:
- removing the native-envelope surrounding a capsid from the virus;
- isolating the previously-enveloped-capsid;
- and
- applying an artificial coating to the previously-enveloped-capsid.
A composition comprising;
- a capsid from a native envelope-virus, wherein the capsid is devoid of its native envelope;
- and
- an artificial coating-layer, wherein the coating-layer encapsulates the capsid.
The surface-engineered delivery system of clam 60, wherein the payload is selected from a recombinant virus or a nucleic acid.
Dependencies are inferred from the claim text.