Aptamers that target cxcl9
AssigneesRheinische Friedrich-Wilhelms-Universität Bonn, Leibniz Universitat Hannover
InventorsGünter MAYER, Julia SIEGL, Cornelia BLUME, Linh PHUNG
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Abstract
The present invention relates to an aptamer comprising a nucleotide sequence SEQ ID NO: 1, preferably comprising or consisting of a nucleotide sequence SEQ ID NO: 4-6. The invention further relates to a composition comprising the aptamer, and the use of the aptamer as a medicament or a diagnostic reagent, particularly for use in the detection or diagnosing of a rejection of a renal allograft.
Claims20
An aptamer comprising a nucleotide sequence 5′-N1N2GN3CCN1AN4N5N1N6N7N8N1-3′ (SEQ ID NO:
- 1) or pharmaceutically acceptable salts thereof, wherein:
- N1 represents T or 5-ethynyl-2′-deoxyuridine (EdU) which is modified with a side chain selected from the group consisting of an indole, benzofurane, naphthalene, phenol, benzothiophene, guanidine and benzyl,
- N2 represents a sequence of 9 or 11 contiguous nucleotides comprising at least 5 guanidine nucleotides,
- N3 represents A or C,
- N4 represents A or a deletion,
- N5 represents C or G,
- N6 represents C or a deletion,
- N7 represents C or G,
- N8 represents G or N1.
An aptamer comprising a nucleotide sequence selected from the group consisting of SEQ ID NO:
- 1, SEQ ID NO:
- 2, SEQ ID NO:
- 3, SEQ ID NO:
- 4, SEQ ID NO:
- 5, SEQ ID NO:
- 6, SEQ ID NO:
- 7, SEQ ID NO:
- 8, SEQ ID NO:
- 9 and pharmaceutically acceptable salts thereof, wherein the aptamer is used as a medicament or a diagnostic reagent.
(canceled)
An in vitro method of detecting or diagnosing a rejection of a renal allograft, the method comprising the steps of:
- 1) selecting an aptamer comprising a nucleotide sequence selected from the group consisting of SEQ ID NO:
- 1, SEQ ID NO:
- 2, SEQ ID NO:
- 3, SEQ ID NO:
- 4, SEQ ID NO:
- 5, SEQ ID NO:
- 6, SEQ ID NO:
- 7, SEQ ID NO:
- 8, SEQ ID NO:
- 9 and pharmaceutically acceptable salts thereof
- 2) detecting the binding of the aptamer to CXCL9 or CXCL11 in a sample obtained from a subject.
Dependencies are inferred from the claim text.