Microarray based sample detection system
AssigneeAkonni Biosystems, Inc.
InventorsChristopher G. COONEY, Peter Qiang QU, Alexander PEROV, Jennifer PARKER
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Abstract
A microarray assembly for detection of a target molecule is disclosed. The microarray assemblies comprise an array chamber having a microarray located therein and features that facilitate liquid movement within the array chamber. Also disclosed are methods for making the microarray assembly using rollable films and methods for detecting microarray spots using an internal control fluorophore in the array spot.
Claims25
A microarray assembly for detection of a target molecule in a sample, comprising:
- an array chamber with a sample inlet at a first end, a sample outlet at a second end, a top interior surface, a bottom interior surface, side walls and a microarray located on the bottom interior surface;
- and
- a waste chamber that is in fluid communication with the outlet of the array chamber,
- wherein the array chamber comprises a hydrophilic interior surface positioned to facilitate complete filling of the array chamber by a water-based fluid and the continuous flow of the fluid from the sample inlet to the sample outlet and wherein the cross-sectional area at the first end of the array chamber is larger than the cross-sectional area at the second end of the array chamber.
A microarray assembly for detection of a target molecule in a sample, comprising:
- an array chamber with a sample inlet, a sample outlet, a top interior surface, a bottom interior surface, side walls and a microarray located on the bottom interior surface;
- a waste chamber comprising a waste inlet and an absorbent material;
- and
- a channel having an expansion section with a first end proximate to the outlet of the array chamber and a second end proximate to the inlet of the waste chamber,
- wherein the top interior surface is a hydrophilic surface that facilitates complete filling of the array chamber by an aqueous fluid and wherein the cross-sectional area at the first end of the expansion section is smaller than the cross-sectional area at the second end of the expansion section.
A microarray assembly for detection of a target molecule in a sample, comprising:
- an array chamber with a sample inlet at a first end, a sample outlet at a second end, a top interior surface, a bottom interior surface, side walls and a microarray located on the bottom surface;
- and
- a waste chamber that is in fluid communication with the outlet of the array chamber,
- wherein the array chamber comprises a hydrophilic interior surface positioned to facilitate complete filling of the array chamber by an aqueous-based fluid and channels with rectangular cross-sectional areas patterned onto the bottom interior surface and/or the top interior surfaces to promote drying.
A microarray assembly for detection of a target molecule in a sample, comprising:
- an array chamber with a sample inlet, a sample outlet, a top surface, a bottom surface, side walls;
- and
- a gel spot microarray located on the bottom surface of the array chamber;
- wherein the gel spot array comprises a plurality of gel spots and wherein each gel spot comprises an internal control fluorophore and a capturing agent that binds specifically to the target molecule.
A method for controlling the quality of manufacturing array elements in a microarray, comprising:
- illuminating a microarray having a plurality of array spots with light waves to produce fluorescence from each array spot;
- measuring fluorescence intensity for each array spot wherein the fluorescence is produced by an internal quality control fluorophore;
- producing a fluorescent image of the microarray;
- determining information for each array spot based on the fluorescent image;
- and
- encoding the information in a barcode, memory device or RFID tag, wherein the barcode, memory device or RFID tag is associated with the microarray.
A method for making a microarray assembly, comprising:
- unrolling a substrate film by one or more substrate film reels;
- printing microarrays onto the unrolled substrate film;
- laminating a spacer film on top of the printed substrate film, wherein the spacer film is pre-cut to provide space for an array chamber prior to the placing step and is placed on top of the printed substrate film by one or more spacer film reels;
- laminating a cover film on top of the spacer film to form a layered microarray structure;
- and
- cutting the layered microarray structure into individual microarray assemblies.
Dependencies are inferred from the claim text.