Variant of inner membrane protein and method for producing target product by using same
AssigneeCj Cheiljedang Corporation
InventorsSeo Yun Kim, Seung Hyun Cho, Jae Min Lee, Min Ji Baek
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Abstract
The present disclosure relates to a variant of YjeH, which is an inner membrane protein, a microorganism including the same, and a method for producing a target product using the same.
Claims16
A variant of an inner membrane protein YjeH comprising i) substitution of an amino acid corresponding to the 92nd position with asparagine, ii) substitution of an amino acid corresponding to the 351st position with leucine, or iii) substitution of both i) and ii), in an amino acid sequence of SEQ ID NO:
- 1, and having a homology of 95% or more and less than 100% with SEQ ID NO:
- 1.
A microorganism comprising at least any one of:
- a variant of an inner membrane protein YjeH comprising i) substitution of an amino acid corresponding to the 92nd position with asparagine, ii) substitution of an amino acid corresponding to the 351st position with leucine, or iii) substitution of both i) and ii), in an amino acid sequence of SEQ ID NO:
- 1, and having a homology of 95% or more and less than 100% with SEQ ID NO:
- 1;
- and a polynucleotide encoding the variant.
A method for producing a target product, the method comprising culturing a microorganism in a culture medium, wherein the microorganism comprises at least any one of:
- a variant of an inner membrane protein YjeH comprising i) substitution of an amino acid corresponding to the 92nd position with asparagine, ii) substitution of an amino acid corresponding to the 351st position with leucine, or iii) substitution of both i) and ii), in an amino acid sequence of SEQ ID NO:
- 1, and having a homology of 95% or more and less than 100% with SEQ ID NO:
- 1;
- and a polynucleotide encoding the variant.
A method for producing methionine, the method comprising:
- producing O-acetylhomoserine by culturing a microorganism in a culture medium, the microorganism comprising at least any one of:
- a variant of an inner membrane protein YjeH comprising i) substitution of an amino acid corresponding to the 92nd position with asparagine, ii) substitution of an amino acid corresponding to the 351st position with leucine, or iii) substitution of both i) and ii), in an amino acid sequence of SEQ ID NO:
- 1, and having a homology of 95% or more and less than 100% with SEQ ID NO:
- 1;
- and a polynucleotide encoding the variant;
- and
- converting the O-acetylhomoserine into methionine by reacting the O-acetylhomoserine with a sulfide.
A method for producing glufosinate, the method comprising:
- producing O-acetylhomoserine or homoserine by culturing a microorganism in a culture medium, the microorganism comprising at least any one of:
- a variant of an inner membrane protein YjeH comprising i) substitution of an amino acid corresponding to the 92nd position with asparagine, ii) substitution of an amino acid corresponding to the 351st position with leucine, or iii) substitution of both i) and ii), in an amino acid sequence of SEQ ID NO:
- 1, and having a homology of 95% or more and less than 100% with SEQ ID NO:
- 1;
- and a polynucleotide encoding the variant;
- and
- converting the O-acetylhomoserine or homoserine into glufosinate.
Dependencies are inferred from the claim text.