Heterologous proteins with axonemal proteins
AssigneeThe Regents of the University of California
InventorsWallace F. MARSHALL, Hongmin QIN
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Abstract
Provided herein are compositions and methods for the production of heterologous proteins in a cell including axonemes. The cells include a nucleic acid encoding a fusion protein expressed in the axoneme. The fusion protein includes an axonemal protein linked to a heterologous protein, and the fusion protein provides axonemal function to the cell.
Claims55
An algal cell comprising:
- a nucleic acid encoding a fusion protein, wherein the fusion protein comprises an axonemal protein linked to a heterologous protein through an enzyme cleavable linker, and wherein the fusion protein provides axonemal function to the algal cell.
A plurality of algal cells wherein each algal cell comprises:
- a nucleic acid encoding a fusion protein, wherein the fusion protein comprises an axonemal protein linked to a heterologous protein through an enzyme cleavable linker, and wherein the fusion protein provides axonemal function to the algal cell.
A recombinant protein comprising:
- a. an algal axonemal protein;
- and
- b. a heterologous protein;
- and
- wherein the axonemal protein is linked to the heterologous protein through an enzyme cleavable linker;
- and
- wherein the recombinant protein provides axonemal function to the algal cell.
An isolated algal flagella comprising:
- a flagellar membrane encompassing an axoneme, wherein said axoneme comprises a fusion protein, wherein the fusion protein comprises an algal axonemal protein linked to a heterologous protein, and wherein the fusion protein provides axonemal function to the algal cell.
An isolated axoneme comprising a fusion protein, wherein said fusion protein comprises an algal axonemal protein linked to a heterologous protein, and wherein the fusion protein provides axonemal function to the algal cell.
A plurality of axonemes wherein each of said plurality of axonemes comprise a fusion protein, wherein said fusion protein comprises an algal axonemal protein linked to a heterologous protein, and wherein the fusion protein provides axonemal function to the algal cell.
A method of isolating a heterologous protein comprising:
- a. expressing a fusion protein in an algal flagella of an algal cell, wherein the fusion protein comprises an algal axonemal protein linked to a heterologous protein through a cleavable linker;
- b. separating said fusion protein from said algal flagella;
- and
- c. contacting said fusion protein with an enzyme thereby isolating said heterologous protein.
Dependencies are inferred from the claim text.