A method for preparing porcine myoglobin using escherichia coli
AssigneeIntron Biotechnology, Inc.
InventorsSeong Jun YOON, Sang Hyeon KANG, Soo Youn JUN, An Sung KWON, Eun Ji LEE
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Abstract
A method for preparing a porcine myoglobin includes: constructing a first plasmid containing genes for heme biosynthesis pathway enzymes; constructing a second plasmid containing a gene for Sus scrofa myoglobin MYG; constructing a first Escherichia coli production host containing the first plasmid and the second plasmid; and producing the porcine myoglobin by culturing the first Escherichia coli production host. A composition useful as a meat flavor and/or an iron supplement includes the porcine myoglobin prepared in accordance with the method.
Claims18
A method for preparing a porcine myoglobin comprising:
- constructing a first plasmid containing genes for heme biosynthesis pathway enzymes;
- constructing a second plasmid containing a gene for Sus scrofa myoglobin MYG;
- constructing a first Escherichia coli production host containing the first plasmid and the second plasmid;
- and
- producing the porcine myoglobin by culturing the first Escherichia coli production host.
A method for preparing a porcine myoglobin comprising:
- constructing a third plasmid containing genes for heme biosynthesis pathway enzymes;
- constructing a second Escherichia coli production host containing the third plasmid;
- and
- producing the porcine myoglobin by culturing the second Escherichia coli production host.
A method for preparing a porcine myoglobin comprising:
- constructing a second plasmid containing a gene for Sus scrofa myoglobin MYG;
- constructing a third Escherichia coli production host containing the second plasmid;
- producing a globin by culturing the third Escherichia coli production host;
- producing a heme by microbial fermentation or chemical synthesis;
- and
- coupling of the globin and the heme to obtain the porcine myoglobin.
Dependencies are inferred from the claim text.