Gene fusions for control of genetically modified cells
AssigneeA2 Biotherapeutics, Inc.
InventorsJee Young Mock, Carl Alexander Kamb, Agnes Hamburger
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Abstract
The disclosure relates to fusion proteins comprising a tBID polypeptide and a steroid hormone receptor domain, and methods of using same to induce apoptosis in cells.
Claims40
A polynucleotide comprising a polynucleotide sequence encoding a fusion protein, the fusion protein comprising a truncated BH3-interacting domain death agonist (tBID) polypeptide and a steroid hormone receptor (SHR) domain polypeptide, wherein the tBID polypeptide comprises a deletion of BID helix 1 and BID helix 2.
The polynucleotide of claim 2, wherein the tBID polypeptide comprises a deletion of BID helix 8.
A polypeptide encoded by the polynucleotide of any one of the preceding claims.
A method of making an immune cell of 29, comprising
- (a) providing a plurality of immune cells;
- and
- (b) transforming the immune cells with a vector encoding a polynucleotide comprising a polynucleotide sequence encoding a fusion protein, the fusion protein comprising a truncated BH3-interacting domain death agonist (tBID) polypeptide and a steroid hormone receptor (SHR) domain polypeptide, wherein the tBID polypeptide comprises a deletion of BID helix 1 and BID helix 2.
Dependencies are inferred from the claim text.