Effector proteins and methods of use

A composition comprising:

• a) an engineered polypeptide or a nucleic acid encoding the engineered polypeptide, wherein the engineered polypeptide comprises an amino acid sequence that is at least 95% identical to SEQ ID NO:
• 1, and wherein the engineered polypeptide comprises at least one amino acid substitution relative to SEQ ID NO:
• 1;
• and
• b) a guide nucleic acid comprising a first sequence and a second sequence,
• wherein the engineered polypeptide is capable of binding the first sequence, and wherein the first sequence and the second sequence are heterologous.

An engineered polypeptide comprising an amino acid sequence that is at least 95% identical to SEQ ID NO:

• 1, wherein the engineered polypeptide comprises at least one amino acid substitution relative to SEQ ID NO:
• 1, and wherein the at least one amino acid substitution occurs at a residue of SEQ ID NO:
• 1 selected from:
• K58, I80, T84, K105, N193, C202, S209, G210, A218, D220, E225, D237, C246, N286, M295, M298, A306, Y315, E335, Q360, D418 or a combination thereof.