Low Diacetyl Yeast
AssigneeCarlsberg A/S
InventorsKlaus Lengeler, Michael Katz, Jochen Förster, Ross Fennessy, Claes Gjermansen, Anna Chailyan
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Abstract
The present invention relates to yeast strains of the species Saccharomyces pastorianus with the useful characteristic of producing low levels of diacetyl during fermentation. Also provided are methods of producing a malt and/or cereal based beverage with these strains, as well as beverages produced by this method.
Claims28
A yeast strain encoding within its genome
- a. no more than two functional genes encoding ILV2, wherein each gene encoding ILV2 encodes ScILV2 of SEQ ID NO:
- 32 or SeILV2 of SEQ ID NO:
- 38, or a functional homologue sharing at least 80% sequence identity with SEQ ID NO:
- 32 or 38;
- and
- b. at least five functional genes encoding ILV3, wherein each gene encoding ILV3 encodes ScILV3 of SEQ ID NO:
- 35 or SeILV3 of SEQ ID NO:
- 41, or a functional homologue sharing at least 80% sequence identity with SEQ ID NO:
- 35 or 41.
(canceled)
8-11. (canceled)
The yeast strain according to claim 61, wherein the sum of the functional genes encoding ILV2 and the functional genes encoding ILV6 is lower than the sum of the functional genes encoding ILV5 and the functional genes encoding ILV3.
The yeast strain according to claim 61, wherein the ratio of the sum of the functional genes encoding ILV5 and the functional gene encoding ILV3 to the functional genes encoding ILV2 is at least 1, and/or
- wherein the ratio of the sum of the functional genes encoding ILV5 and the functional genes encoding ILV3 to the sum of the functional gene encoding ILV2 and the functional encoding ILV6 is at least 1.
(canceled)
-20. (canceled)
-35. (canceled)
(canceled)
-51. (canceled)
-54. (canceled)
Dependencies are inferred from the claim text.