Use of Gene Encoding Gibberellin 3Beta-Hydroxylase of Glycine Max, GmGA3ox1
AssigneeNanjing Agricultural University
InventorsDeyue YU, Fang HUANG, Dezhou HU, Hui WANG, Zhongyi YANG, Shaoqi LU, Xiao LI
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Abstract
The present disclosure discloses the use of gene encoding gibberellin 3β-hydroxylase of G. max, GmGA3ox1. The use of gibberellin 3β-hydroxylase gene of G. max, GmGA3ox1, set forth in SEQ ID NO:1, in genetic engineering of seed weight of Arabidopsis thaliana and Glycine max. In A. thaliana, overexpression of GmGA3ox1 can complement the low seed weight phenotype of atga3ox1 mutant. In G. max, overexpression of the excellent haplotype of the gene can significantly improve the seed weight of G. max.
Claims10
A method for genetic engineering of seed weight of Arabidopsis thaliana and seed weight or length of Glycine max, comprising using gibberellin 3β-hydroxylase of Glycine max or GmGA3ox1 gene encoding gibberellin 3β-hydroxylase of Glycine max, wherein the amino acid sequence of the gibberellin 3β-hydroxylase of Glycine max is set forth in SEQ ID NO:
- 2 and the sequence of GmGA3ox1 gene encoding gibberellin 3β-hydroxylase of Glycine max is set forth in SEQ ID NO:
- 1.
A method for improving the yield of Arabidopsis thaliana or Glycine max comprising overexpressing the gibberellin 3β-hydroxylase of Glycine max or GmGA3ox1 gene encoding gibberellin 3β-hydroxylase of Glycine max, in Arabidopsis thaliana or Glycine max, wherein the amino acid sequence of the gibberellin 3β-hydroxylase of Glycine max is set forth in SEQ ID NO:
- 2 and the sequence of GmGA3ox1 gene encoding gibberellin 3β-hydroxylase of Glycine max is set forth in SEQ ID NO:1.
A primer pair for detecting the expression level of the GmGA3ox1 gene encoding gibberellin 3β-hydroxylase of Glycine max, wherein the nucleotide sequences of the primer pair are set forth in SEQ ID NO:
- 7 and SEQ ID NO:
- 8.
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Dependencies are inferred from the claim text.