Modified filamentous fungi for production of exogenous proteins
AssigneeDyadic International (USA), Inc.
InventorsMarika Vitikainen, Anne Huuskonen, Andriy Kovalchuk, Christopher Landowski, Ronen Tchelet, Mark Aaron Emalfarb, Markku Saloheimo
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Abstract
The present invention relates to genetically modified ascomycetous filamentous fungi, particularly of the species Thermothelomyces heterothallica, having reduced activity or expression of KEX2 and/or ALP7, said filamentous fungi is capable of producing elevated amounts and stability of an exogenous protein.
Claims34
A genetically modified ascomycetous filamentous fungus for producing an exogenous protein of interest, the genetically modified filamentous fungus comprises at least one cell having reduced expression and activity of at least one protease selected from KEX2 and ALP7, the at least one cell comprising an exogenous polynucleotide encoding the protein of interest.
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A method for producing a fungus capable of producing a protein of interest, the method comprising transforming at least one cell of the fungus with at least one exogenous polynucleotide encoding the protein of interest;
- said at least one cell of the fungus having reduced expression and/or activity of at least one protease selected from KEX2 and/or ALP7.
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Dependencies are inferred from the claim text.