Devices and Methods to Induce Adult type Maturation of Human Pluripotent Stem Cell Derived Cardiomyocytes

A construct, comprising:

• (a) a patterned scaffold at submicron resolution, wherein the patterned scaffold comprises a polymeric hydrogel substrate comprising a plurality of wrinkles, wherein the wrinkles comprise linear or branched folds directionally aligned over a centimeter length scale, wherein the polymeric substrate has a viscoelasticity between about 15 kPa and about 100 MPa, or about 15 kPa to about 75 MPa;
• (b) one or more cardiac matrix ligands conjugated to the patterned scaffold, wherein the one or more cardiac matrix ligands comprises 1, 2, 3, 4 or more of Nephronectin (SEQ ID NO:
• 13), GRGDS (SEQ ID NO:
• 10), GFOGER (SEQ ID NO:
• 11), GFPGER (SEQ ID NO:
• 12) and/or other peptides containing one or more RGD motifs.