Biological Detection Sensor Chip and COVID-19 Test Kit

Abstract

The invention discloses a biosensor chip and a COVID-19 test kit. The COVID-19 test kit includes a biosensor chip, rolling circle amplification (RCA) primers, RCA padlock probes, and ssDNA conjugated nanoparticles (i.e., ssDNA-NPs probe). In this invention, the surface of the biosensor chip is modified with functional polymers; subsequently, detection ligands are grafted on the modified sensor chip surface to capture the targets. The developed biosensor chip achieves rapid and ultra-sensitive on-site detection of COVID-19 infections. In addition, a signal amplification method is employed using RCA products hybridized ssDNA-NPs probes (i.e., RCA-NPs) complex to amplify the detection signal and to improve the detection sensitivity. The RCA sequence in this invention is designed to generate tandem repeating aptamers and ssDNA-NPs probe hybridization sites so that multiple ssDNA-NPs probes can be hybridized with the RCA products and attached to the captured targets in order to increase the target mass, resulting in detection signal intensification.

Dependencies are inferred from the claim text.