Compositions to detect adenovirus nucleic acids
AssigneeGen-Probe Prodesse, Inc.
InventorsEmily ZIEGLER, Jessica TOWNSEND
Life of this patent
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Abstract
The disclosed invention is related to methods, compositions, kits and isolated nucleic acid sequences for targeting Adenovirus nucleic acid. Compositions include amplification oligomers and/or detection probe oligomers. Kits and methods comprise at least one of these oligomers.
Claims20
A composition comprising a detection probe configured to hybridize to a portion of an Adenovirus nucleic acid or to a portion of an amplicon generated therefrom, wherein the detection probe comprises
- (a) a target hybridizing sequence selected from the group consisting of SEQ ID NO:10, SEQ ID NO:17, SEQ ID NO:18, SEQ ID NO:19, SEQ ID NO:20, SEQ ID NO:21, SEQ ID NO:22, SEQ ID NO:23, SEQ ID NO:24, SEQ ID NO:29, SEQ ID NO:30, SEQ ID NO:36, SEQ ID NO:37, SEQ ID NO:39 and SEQ ID NO:40;
- and
- (b) one or more of
- (i) a label,
- (ii) a substitution at the 2′ position of at least one ribose moiety, and
- (iii) a blocking moiety at or near the 3′ end of the detection probe, wherein the blocking moiety is configured to prevent enzyme-mediated extension of the detection probe in an amplification reaction.
A composition comprising at least two detection probes configured to hybridize to a portion of an Adenovirus nucleic acid or to a portion of an amplicon generated therefrom, wherein each of the detection probes comprises
- (a) a target hybridizing sequence selected from the group consisting of SEQ ID NO:10, SEQ ID NO:17, SEQ ID NO:18, SEQ ID NO:19, SEQ ID NO:20, SEQ ID NO:21, SEQ ID NO:22, SEQ ID NO:23, SEQ ID NO:24, SEQ ID NO:29, SEQ ID NO:30, SEQ ID NO:36, SEQ ID NO:37, SEQ ID NO:39 and SEQ ID NO:40;
- and
- (b) one or more of
- (i) a label,
- (ii) a substitution at the 2′ position of at least one ribose moiety, and
- (iii) a blocking moiety at or near the 3′ end of the detection probe, wherein the blocking moiety is configured to prevent enzyme-mediated extension of the detection probe in an amplification reaction.
A hybridization assay reaction mixture comprising:
- (1) a detection probe configured to hybridize to a portion of an Adenovirus nucleic acid or to a portion of an amplicon generated therefrom, wherein the detection probe comprises
- (a) a target hybridizing sequence selected from the group consisting of SEQ ID NO:10, SEQ ID NO:17, SEQ ID NO:18, SEQ ID NO:19, SEQ ID NO:20, SEQ ID NO:21, SEQ ID NO:22, SEQ ID NO:23, SEQ ID NO:24, SEQ ID NO:29, SEQ ID NO:30, SEQ ID NO:36, SEQ ID NO:37, SEQ ID NO:39 and SEQ ID NO:40;
- and
- (b) one or more of
- (i) a label,
- (ii) a substitution at the 2′ position of at least one ribose moiety, and
- (iii) a blocking moiety at or near the 3′ end of the detection probe, wherein the blocking moiety is configured to prevent enzyme-mediated extension of the detection probe in an amplification reaction;
- and
- (2) a salt.
Dependencies are inferred from the claim text.