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US 2023/0292813 A1Published application
Tobacco-derived colorants and colored substrates
AssigneeR.J. Reynolds Tobacco Company
InventorsCharles Stewart, Ercilia Hernandez Garcia, Robert Owen Bussey, III
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Abstract
Methods for obtaining natural colorants from plant material (e.g., tobacco material) and for using such natural colorants to dye various substrates are provided. Natural colorants are obtained using particular enzymes and particular conditions (e.g., time, temperature, and pH profiles). Such colorants can be used to dye substrates, for example, using conventional dyeing techniques or using unique in situ methods.
Claims21
A method of extracting colorant from a tobacco material, the method comprising:
- providing a first tobacco mixture by combining the tobacco material with a cellulase and a beta glucosidase at a pH of about 5-6;
- providing a second tobacco mixture by adjusting the pH of the first tobacco mixture to a pH of about 7-8 and adding pectate lyase and xylanase to the first tobacco mixture;
- and
- separating the tobacco material from the second tobacco mixture to give a colorant-containing broth.
1 more element
21-23. (canceled)
Dependencies are inferred from the claim text.