US 2023/0287464 A1 · MICROORGANISMS AND METHODS FOR THE PRODUCTION OF BUTADIENE USING ACETYL-COA

A non-naturally occurring microbial organism, said microbial organism having a butadiene pathway and comprising at least one exogenous nucleic acid encoding a butadiene pathway enzyme expressed in a sufficient amount to produce butadiene, wherein said butadiene pathway comprises a pathway selected from:

(1) 1A, 1B, 1C, 1G, 1I, 1L, 1M, and 1F;
(2) 1A, 1B, 1C, 1G, 1I, 1L, 1N, and 1F;
(3) 1A, 1B, 1C, 1H, 1I, 1L, 1M, and 1F;
(4) 1A, 1B, 1C, 1H, 1I, 1L, 1N, and 1F;
(5) 1A, 1B, 1C, 1D, 1J, 1L, 1M, and 1F;
(6) 1A, 1B, 1C, 1D, 1J, 1L, 1N, and 1F;
(7) 1A, 1B, 1C, 1D, 1K, 1L, 1M, and 1F;
(8) 1A, 1B, 1C, 1D, 1K, 1L, 1N, and 1F;
(9) 1B, 1C, 1G, 1I, 1L, 1M, and 1F;
(10) 1B, 1C, 1G, 1I, 1L, 1N, and 1F;
(11) 1B, 1C, 1H, 1I, 1L, 1M, and 1F;
(12) 1B, 1C, 1H, 1I, 1L, 1N, and 1F;
(13) 1B, 1C, 1D, 1J, 1L, 1M, and 1F;
(14) 1B, 1C, 1D, 1J, 1L, 1N, and 1F;
(15) 1B, 1C, 1D, 1K, 1L, 1M, and 1F;
(16) 1B, 1C, 1D, 1K, 1L, 1N, and 1F;
(17) 2A, 2B, 2C, 2R, 2S, 2E, 2J, 2L, 2O, 2Q, and 2I;
(18) 2A, 2B, 2C, 2R, 2S, 2E, 2J, 2L, 2O, 2P, and 2I;
(19) 2A, 2B, 2C, 2R, 2S, 2E, 2K, 2L, 2O, 2Q, and 2I;
(20) 2A, 2B, 2C, 2R, 2S, 2E, 2K, 2L, 2O, 2P, and 2I;
(21) 2A, 2B, 2C, 2R, 2S, 2E, 2G, 2M, 2O, 2Q, and 2I;
(22) 2A, 2B, 2C, 2R, 2S, 2E, 2G, 2M, 2O, 2P, and 2I;
(23) 2A, 2B, 2C, 2R, 2S, 2E, 2G, 2N, 2O, 2Q, and 2I;
(24) 2A, 2B, 2C, 2R, 2S, 2E, 2G, 2N, 2O, 2P, and 2I;
(25) 2A, 2B, 2C, 2R, 2S, 2E, 2G, 2H, and 2I;
(26) 2A, 2B, 2C, 2D, 2E, 2J, 2L, 2O, 2Q, and 2I;
(27) 2A, 2B, 2C, 2D, 2E;
2J, 2L, 2O, 2P, and 2I;
(28) 2A, 2B, 2C, 2D, 2E, 2K, 2L, 2O, 2Q, and 2I;
(29) 2A, 2B, 2C, 2D, 2E, 2K, 2L, 2O, 2P, and 2I;
(30) 2A, 2B, 2C, 2D, 2E, 2G, 2M, 2O, 2Q, and 2I;
(31) 2A, 2B, 2C, 2D, 2E, 2G, 2M, 2O, 2P, and 2I;
(32) 2A, 2B, 2C, 2D, 2E, 2G, 2N, 2O, 2Q, and 2I;
(33) 2A, 2B, 2C, 2D, 2E, 2G, 2N, 2O, 2P, and 2I;
(34) 2A, 2B, 2C, 2F, 2J, 2L, 2O, 2Q, and 2I;
(35) 2A, 2B, 2C, 2F, 2J, 2L, 2O, 2P, and 2I;
(36) 2A, 2B, 2C, 2F, 2K, 2L, 2O, 2Q, and 2I;
(37) 2A, 2B, 2C, 2F, 2K, 2L, 2O, 2P, and 2I;
(38) 2A, 2B, 2C, 2F, 2G, 2M, 2O, 2Q, and 2I;
(39) 2A, 2B, 2C, 2F, 2G, 2M, 2O, 2P, and 2I;
(40) 2A, 2B, 2C, 2F, 2G, 2N, 2O, 2Q, and 2I;
(41) 2A, 2B, 2C, 2F, 2G, 2N, 2O, 2P, and 2I;
(42) 2B, 2C, 2R, 2S, 2E, 2J, 2L, 2O, 2Q, and 2I;
(43) 2B, 2C, 2R, 2S, 2E, 2J, 2L, 2O, 2P, and 2I;
(44) 2B, 2C, 2R, 2S, 2E, 2K, 2L, 2O, 2Q, and 2I;
(45) 2B, 2C, 2R, 2S, 2E, 2K, 2L, 2O, 2P, and 2I;
(46) 2B, 2C, 2R, 2S, 2E, 2G, 2M, 2O, 2Q, and 2I;
(47) 2B, 2C, 2R, 2S, 2E, 2G, 2M, 2O, 2P, and 2I;
(48) 2B, 2C, 2R, 2S, 2E, 2G, 2N, 2O, 2Q, and 2I;
(49) 2B, 2C, 2R, 2S, 2E, 2G, 2N, 2O, 2P, and 2I;
(50) 2B, 2C, 2R, 2S, 2E, 2G, 2H, and 2I;
(51) 2B, 2C, 2D, 2E, 2J, 2L, 2O, 2Q, and 2I;
(52) 2B, 2C, 2D, 2E, 2J, 2L, 2O, 2P, and 2I;
(53) 2B, 2C, 2D, 2E, 2K, 2L, 2O, 2Q, and 2I;
(54) 2B, 2C, 2D, 2E, 2K, 2L, 2O, 2P, and 2I;
(55) 2B, 2C, 2D, 2E, 2G, 2M, 2O, 2Q, and 2I;
(56) 2B, 2C, 2D, 2E, 2G, 2M, 2O, 2P, and 2I;
(57) 2B, 2C, 2D, 2E, 2G, 2N, 2O, 2Q, and 2I;
(58) 2B, 2C, 2D, 2E, 2G, 2N, 2O, 2P, and 2I;
(59) 2B, 2C, 2F, 2J, 2L, 2O, 2Q, and 2I;
(60) 2B, 2C, 2F, 2J, 2L, 2O, 2P, and 2I;
(61) 2B, 2C, 2F, 2K, 2L, 2O, 2Q, and 2I;
(62) 2B, 2C, 2F, 2K, 2L, 2O, 2P, and 2I;
(63) 2B, 2C, 2F, 2G, 2M, 2O, 2Q, and 2I;
(64) 2B, 2C, 2F, 2G, 2M, 2O, 2P, and 2I;
(65) 2B, 2C, 2F, 2G, 2N, 2O, 2Q, and 2I;
(66) 2B, 2C, 2F, 2G, 2N, 2O, 2P, and 2I,
(67) 1C, 1G, 1I, 1L, 1M, and 1F;
(68) 1C, 1G, 1I, 1L, 1N, and 1F;
(69) 1C, 1H, 1I, 1L, 1M, and 1F;
(70) 1C, 1H, 1I, 1L, 1N, and 1F;
(71) 1C, 1D, 1J, 1L, 1M, and 1F;
(72) 1C, 1D, 1J, 1L, 1N, and 1F;
(73) 1C, 1D, 1K, 1L, 1M, and 1F;
(74) 1C, 1D, 1K, 1L, 1N, and 1F;
(75) 2C, 2R, 2S, 2E, 2J, 2L, 2O, 2Q, and 2I;
(76) 2C, 2R, 2S, 2E, 2J, 2L, 2O, 2P, and 2I;
(77) 2C, 2R, 2S, 2E, 2K, 2L, 2O, 2Q, and 2I;
(78) 2C, 2R, 2S, 2E, 2K, 2L, 2O, 2P, and 2I;
(79) 2C, 2R, 2S, 2E, 2G, 2M, 2O, 2Q, and 2I;
(80) 2C, 2R, 2S, 2E, 2G, 2M, 2O, 2P, and 2I;
(81) 2C, 2R, 2S, 2E, 2G, 2N, 2O, 2Q, and 2I;
(82) 2C, 2R, 2S, 2E, 2G, 2N, 2O, 2P, and 2I;
(83) 2C, 2R, 2S, 2E, 2G, 2H, and 2I;
(84) 2C, 2D, 2E, 2J, 2L, 2O, 2Q, and 2I;
(85) 2C, 2D, 2E, 2J, 2L, 2O, 2P, and 2I;
(86) 2C, 2D, 2E, 2K, 2L, 2O, 2Q, and 2I;
(87) 2C, 2D, 2E, 2K, 2L, 2O, 2P, and 2I;
(88) 2C, 2D, 2E, 2G, 2M, 2O, 2Q, and 2I;
(89) 2C, 2D, 2E, 2G, 2M, 2O, 2P, and 2I;
(90) 2C, 2D, 2E, 2G, 2N, 2O, 2Q, and 2I;
(91) 2C, 2D, 2E, 2G, 2N, 2O, 2P, and 2I;
(92) 2C, 2F, 2J, 2L, 2O, 2Q, and 2I;
(93) 2C, 2F, 2J, 2L, 2O, 2P, and 2I;
(94) 2C, 2F, 2K, 2L, 2O, 2Q, and 2I;
(95) 2C, 2F, 2K, 2L, 2O, 2P, and 2I;
(96) 2C, 2F, 2G, 2M, 2O, 2Q, and 2I;
(97) 2C, 2F, 2G, 2M, 2O, 2P, and 2I;
(98) 2C, 2F, 2G, 2N, 2O, 2Q, and 2I;
and
(99) 2C, 2F, 2G, 2N, 2O, 2P, and 2I,
wherein 1A is an acetaldehyde dehydrogenase, wherein 1B is a 4-hydroxy 2-oxovalerate aldolase, wherein 1C is a 4-hydroxy 2-oxovalerate dehydratase, wherein 1D is a 2-oxopentenoate reductase, wherein 1E is a 2-hydroxypentenoate dehydratase, wherein 1F is a 2,4-pentadienoate decarboxylase, wherein 1G is a 2-oxopentenoate ligase, wherein 1H is a 2-oxopentenoate:
acetyl CoA CoA transferase, wherein 1I is a 2-oxopentenoyl-CoA reductase, wherein 1J is a 2-hydroxypentenoate ligase, wherein 1K is a 2-hydroxypentenoate:
acetyl-CoA CoA transferase, wherein 1L is a 2-hydroxypentenoyl-CoA dehydratase, wherein 1M is a 2,4-Pentadienoyl-CoA hydrolase, wherein 1N is a 2,4-Pentadienoyl-CoA:
acetyl CoA CoA transferase, wherein 2A is an acetyl-CoA carboxylase, wherein 2B is a malonyl-CoA:acetyl-CoA acyltransferase, wherein 2C is a 3-Oxoglutaryl-CoA reductase (ketone-reducing), wherein 2D is a 3-hydroxyglutaryl-CoA reductase (aldehyde forming), wherein 2E is a 3-hydroxy-5-oxopentanoate reductase, wherein 2F is a 3-hydroxyglutaryl-CoA reductase (alcohol forming), wherein 2G is a 3,5-dihydroxypentanoate dehydratase, wherein 2H is a 5-hydroxypent-2-enoate dehydratase, wherein 2I is a 2,4-pentadienoate decarboxylase, wherein 2J is a 3,5-dihydroxypentanoate ligase, wherein 2K is a 3,5-dihydroxypentanoate:
acetyl-CoA CoA transferase, wherein 2L is a 3,5-dihydroxypentanoyl-CoA dehydratase, wherein 2M is a 5-hydroxypent-2-enoate ligase, wherein 2N is a 5-hydroxypent-2-enoate:
acetyl-CoA CoA transferase, wherein 2O is a 5-hydroxypent-2-enoyl-CoA hydrolase, wherein 2P is a 2,4-pentadienoyl-CoA CoA hydrolase, wherein 2Q is a 2,4-pentadienoyl-CoA:
acetyl-CoA CoA transferase, wherein 2R is a Phosphate-3-hydroxyglutaryl transferase, and wherein 2S is a 3-hydroxy-5-oxopentanoate synthase.

105 more elements

A non-naturally occurring microbial organism, said microbial organism having a 2,4-pentadienoate pathway and comprising at least one exogenous nucleic acid encoding a 2,4-pentadienoate pathway enzyme expressed in a sufficient amount to produce 2,4-pentadienoate, wherein said 2,4-pentadienoate pathway comprises a pathway selected from:

(1) 1A, 1B, 1C, 1G, 1I, 1L, and 1M;
(2) 1A, 1B, 1C, 1G, 1I, 1L, and 1N;
(3) 1A, 1B, 1C, 1H, 1I, 1L, and 1M;
(4) 1A, 1B, 1C, 1H, 1I, 1L, and 1N;
(5) 1A, 1B, 1C, 1D, 1J, 1L, and 1M;
(6) 1A, 1B, 1C, 1D, 1J, 1L, and 1N;
(7) 1A, 1B, 1C, 1D, 1K, 1L, and 1M;
(8) 1A, 1B, 1C, 1D, 1K, 1L, and 1N;
(9) 1B, 1C, 1G, 1I, 1L, and 1M;
(10) 1B, 1C, 1G, 1I, 1L, and 1N;
(11) 1B, 1C, 1H, 1I, 1L, and 1M;
(12) 1B, 1C, 1H, 1I, 1L, and 1N;
(13) 1B, 1C, 1D, 1J, 1L, and 1M;
(14) 1B, 1C, 1D, 1J, 1L, and 1N;
(15) 1B, 1C, 1D, 1K, 1L, and 1M;
(16) 1B, 1C, 1D, 1K, 1L, and 1N;
(17) 2A, 2B, 2C, 2R, 2S, 2E, 2J, 2L, 2O, and 2Q;
(18) 2A, 2B, 2C, 2R, 2S, 2E, 2J, 2L, 2O, and 2P;
(19) 2A, 2B, 2C, 2R, 2S, 2E, 2K, 2L, 2O, and 2Q;
(20) 2A, 2B, 2C, 2R, 2S, 2E, 2K, 2L, 2O, and 2P;
(21) 2A, 2B, 2C, 2R, 2S, 2E, 2G, 2M, 2O, and 2Q;
(22) 2A, 2B, 2C, 2R, 2S, 2E, 2G, 2M, 2O, and 2P;
(23) 2A, 2B, 2C, 2R, 2S, 2E, 2G, 2N, 2O, and 2Q;
(24) 2A, 2B, 2C, 2R, 2S, 2E, 2G, 2N, 2O, and 2P;
(25) 2A, 2B, 2C, 2R, 2S, 2E, 2G, and 2H;
(26) 2A, 2B, 2C, 2D, 2E, 2J, 2L, 2O, and 2Q;
(27) 2A, 2B, 2C, 2D, 2E;
2J, 2L, 2O, and 2P;
(28) 2A, 2B, 2C, 2D, 2E, 2K, 2L, 2O, and 2Q;
(29) 2A, 2B, 2C, 2D, 2E, 2K, 2L, 2O, and 2P;
(30) 2A, 2B, 2C, 2D, 2E, 2G, 2M, 2O, and 2Q;
(31) 2A, 2B, 2C, 2D, 2E, 2G, 2M, 2O, and 2P;
(32) 2A, 2B, 2C, 2D, 2E, 2G, 2N, 2O, and 2Q;
(33) 2A, 2B, 2C, 2D, 2E, 2G, 2N, 2O, and 2P;
(34) 2A, 2B, 2C, 2F, 2J, 2L, 2O, and 2Q;
(35) 2A, 2B, 2C, 2F, 2J, 2L, 2O, and 2P;
(36) 2A, 2B, 2C, 2F, 2K, 2L, 2O, and 2Q;
(37) 2A, 2B, 2C, 2F, 2K, 2L, 2O, and 2P;
(38) 2A, 2B, 2C, 2F, 2G, 2M, 2O, and 2Q;
(39) 2A, 2B, 2C, 2F, 2G, 2M, 2O, and 2P;
(40) 2A, 2B, 2C, 2F, 2G, 2N, 2O, and 2Q;
(41) 2A, 2B, 2C, 2F, 2G, 2N, 2O, and 2P;
(42) 2B, 2C, 2R, 2S, 2E, 2J, 2L, 2O, and 2Q;
(43) 2B, 2C, 2R, 2S, 2E, 2J, 2L, 2O, and 2P;
(44) 2B, 2C, 2R, 2S, 2E, 2K, 2L, 2O, and 2Q;
(45) 2B, 2C, 2R, 2S, 2E, 2K, 2L, 2O, and 2P;
(46) 2B, 2C, 2R, 2S, 2E, 2G, 2M, 2O, and 2Q;
(47) 2B, 2C, 2R, 2S, 2E, 2G, 2M, 2O, and 2P;
(48) 2B, 2C, 2R, 2S, 2E, 2G, 2N, 2O, and 2Q;
(49) 2B, 2C, 2R, 2S, 2E, 2G, 2N, 2O, and 2P;
(50) 2B, 2C, 2R, 2S, 2E, 2G, and 2H;
(51) 2B, 2C, 2D, 2E, 2J, 2L, 2O, and 2Q;
(52) 2B, 2C, 2D, 2E, 2J, 2L, 2O, and 2P;
(53) 2B, 2C, 2D, 2E, 2K, 2L, 2O, and 2Q;
(54) 2B, 2C, 2D, 2E, 2K, 2L, 2O, and 2P;
(55) 2B, 2C, 2D, 2E, 2G, 2M, 2O, and 2Q;
(56) 2B, 2C, 2D, 2E, 2G, 2M, 2O, and 2P;
(57) 2B, 2C, 2D, 2E, 2G, 2N, 2O, and 2Q;
(58) 2B, 2C, 2D, 2E, 2G, 2N, 2O, and 2P;
(59) 2B, 2C, 2F, 2J, 2L, 2O, and 2Q;
(60) 2B, 2C, 2F, 2J, 2L, 2O, and 2P;
(61) 2B, 2C, 2F, 2K, 2L, 2O, and 2Q;
(62) 2B, 2C, 2F, 2K, 2L, 2O, and 2P;
(63) 2B, 2C, 2F, 2G, 2M, 2O, and 2Q;
(64) 2B, 2C, 2F, 2G, 2M, 2O, and 2P;
(65) 2B, 2C, 2F, 2G, 2N, 2O, and 2Q;
(66) 2B, 2C, 2F, 2G, 2N, 2O, and 2P,
(67) 1C, 1G, 1I, 1L, and 1M;
(68) 1C, 1G, 1I, 1L, and 1N;
(69) 1C, 1H, 1I, 1L, and 1M;
(70) 1C, 1H, 1I, 1L, and 1N;
(71) 1C, 1D, 1J, 1L, and 1M;
(72) 1C, 1D, 1J, 1L, and 1N;
(73) 1C, 1D, 1K, 1L, and 1M;
(74) 1C, 1D, 1K, 1L, and 1N;
(75) 2C, 2R, 2S, 2E, 2J, 2L, 2O, and 2Q;
(76) 2C, 2R, 2S, 2E, 2J, 2L, 2O, and 2P;
(77) 2C, 2R, 2S, 2E, 2K, 2L, 2O, and 2Q;
(78) 2C, 2R, 2S, 2E, 2K, 2L, 2O, and 2P;
(79) 2C, 2R, 2S, 2E, 2G, 2M, 2O, and 2Q;
(80) 2C, 2R, 2S, 2E, 2G, 2M, 2O, and 2P;
(81) 2C, 2R, 2S, 2E, 2G, 2N, 2O, and 2Q;
(82) 2C, 2R, 2S, 2E, 2G, 2N, 2O, and 2P;
(83) 2C, 2R, 2S, 2E, 2G, and 2H;
(84) 2C, 2D, 2E, 2J, 2L, 2O, and 2Q;
(85) 2C, 2D, 2E, 2J, 2L, 2O, and 2P;
(86) 2C, 2D, 2E, 2K, 2L, 2O, and 2Q;
(87) 2C, 2D, 2E, 2K, 2L, 2O, and 2P;
(88) 2C, 2D, 2E, 2G, 2M, 2O, and 2Q;
(89) 2C, 2D, 2E, 2G, 2M, 2O, and 2P;
(90) 2C, 2D, 2E, 2G, 2N, 2O, and 2Q;
(91) 2C, 2D, 2E, 2G, 2N, 2O, and 2P;
(92) 2C, 2F, 2J, 2L, 2O, and 2Q;
(93) 2C, 2F, 2J, 2L, 2O, and 2P;
(94) 2C, 2F, 2K, 2L, 2O, and 2Q;
(95) 2C, 2F, 2K, 2L, 2O, and 2P;
(96) 2C, 2F, 2G, 2M, 2O, and 2Q;
(97) 2C, 2F, 2G, 2M, 2O, and 2P;
(98) 2C, 2F, 2G, 2N, 2O, and 2Q;
and
(99) 2C, 2F, 2G, 2N, 2O, and 2P,
wherein 1A is an acetaldehyde dehydrogenase, wherein 1B is a 4-hydroxy 2-oxovalerate aldolase, wherein 1C is a 4-hydroxy 2-oxovalerate dehydratase, wherein 1D is a 2-oxopentenoate reductase, wherein 1E is a 2-hydroxypentenoate dehydratase, wherein 1G is a 2-oxopentenoate ligase, wherein 1H is a 2-oxopentenoate:
acetyl CoA CoA transferase, wherein 1I is a 2-oxopentenoyl-CoA reductase, wherein 1J is a 2-hydroxypentenoate ligase, wherein 1K is a 2-hydroxypentenoate:
acetyl-CoA CoA transferase, wherein 1L is a 2-hydroxypentenoyl-CoA dehydratase, wherein 1M is a 2,4-Pentadienoyl-CoA hydrolase, wherein 1N is a 2,4-Pentadienoyl-CoA:
acetyl CoA CoA transferase, wherein 2A is an acetyl-CoA carboxylase, wherein 2B is a malonyl-CoA:acetyl-CoA acyltransferase, wherein 2C is a 3-Oxoglutaryl-CoA reductase (ketone-reducing), wherein 2D is a 3-hydroxyglutaryl-CoA reductase (aldehyde forming), wherein 2E is a 3-hydroxy-5-oxopentanoate reductase, wherein 2F is a 3-hydroxyglutaryl-CoA reductase (alcohol forming), wherein 2G is a 3,5-dihydroxypentanoate dehydratase, wherein 2H is a 5-hydroxypent-2-enoate dehydratase, wherein 2J is a 3,5-dihydroxypentanoate ligase, wherein 2K is a 3,5-dihydroxypentanoate:
acetyl-CoA CoA transferase, wherein 2L is a 3,5-dihydroxypentanoyl-CoA dehydratase, wherein 2M is a 5-hydroxypent-2-enoate ligase, wherein 2N is a 5-hydroxypent-2-enoate:
acetyl-CoA CoA transferase, wherein 2O is a 5-hydroxypent-2-enoyl-CoA hydrolase, wherein 2P is a 2,4-pentadienoyl-CoA CoA hydrolase, wherein 2Q is a 2,4-pentadienoyl-CoA:
acetyl-CoA CoA transferase, wherein 2R is a Phosphate-3-hydroxyglutaryl transferase, and wherein 2S is a 3-hydroxy-5-oxopentanoate synthase.

105 more elements

Microorganisms and methods for the production of butadiene using acetyl-coa

Life of this patent

Microorganisms and methods for the production of butadiene using acetyl-coa

Life of this patent

Abstract

Claims36