Methods and compositions for the generation of programable post-translational protein modification and hydrolysis
AssigneesThe Regents of the University of Colorado, a body corporate, The Regents of the University of California
InventorsAaron Whiteley, Hannah Ledvina, Kevin Corbett, Qiaozhen Ye, Yajie Gu
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Abstract
The invention describes the discovery and novel application of a bacterial ubiquitin transferase (Cap2). Specifically, the invention describes the novel activity of the enzyme Cap2 which is capable of creating a specific fusion between two proteins implementing a standalone catalytic mechanism to create the fusion.
Claims26
A system of generating a fusion peptide comprising:
- a first peptide;
- a Cap2 enzyme having a target recognition motif,
- a target peptide coupled with said target recognition motif;
- wherein said Cap2 enzyme ligates said first peptide to said target peptide, forming a fusion peptide.
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An isolated composition comprising:
- a fusion peptide including:
- a first peptide;
- a Cap2 enzyme, or a fragment or variant thereof, having a target recognition motif,
- a target peptide;
- and
- an intermediary peptide.
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Dependencies are inferred from the claim text.