Novel antibacterial peptide and use thereof
AssigneeAnygen Co., Ltd.
InventorsJae Il KIM, Jae Ha RYU, Shang Hyeon KIM, Sol LEE
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Abstract
The present invention relates to a novel antibacterial peptide and a composition comprising the same as an active ingredient. The novel antibacterial peptide of the present invention exhibits excellent antibacterial activity against antibiotic-resistant bacteria as well as Gram-positive bacteria and Gram-negative bacteria and is low in cytotoxicity and, as such, can be advantageously utilized as an active ingredient in an antibiotic, a cosmetic composition, a food additive, a feed additive, a biotic pesticide, a quasi-drug product, and the like.
Claims20
An antibacterial peptide represented by Structural Formula I or Structural Formula II below or a salt thereof:
- in which,
- N′ is the N-terminus of the antibacterial peptide,
- C′ is the C-terminus of the antibacterial peptide,
- the C-terminus is one in which a carboxy group or hydroxy (-OH) of the carboxy group is substituted with an amine group (-NH2),
- A consists of the amino acid sequence of (X1)a-(X2)b-(X3)c-(X4)d,
- wherein a, b, c and d are each independently 0 or 1, and
- X1 to X4 are each independently any one amino acid selected from the group consisting of Arg, Lys, Asn, Gln, Asp, Val, Leu, Ser, His, Gly and Tyr, and
- B consists of the amino acid sequence of Trp-(Z1)e-(Z2)f-Trp-(Z3)g-(Z4)h-Trp,
- wherein e, f, g and h are each independently 0 or 1, and
- Z1 to Z4 are each independently any one amino acid selected from the group consisting of Val, Leu, Ile, Gly, Ala, Ser, Phe, Tyr, Trp, Lys and His, and
- C consists of the amino acid sequence of (X5)i-(X6)j-(X7)k-(X8)1,
- wherein i, j, k and l are each independently 0 or 1, and
- X5 to X8 are each independently any one amino acid selected from the group consisting of Arg, Lys, Asn, Gln, Asp, Val, Leu, Ser, His, Gly and Tyr, and
- wherein Trp may be substituted with C1-6 alkoxy or halogen, or nitrogen (N) in the indole ring of Trp may be modified with sulfur (S), and
- when an amino acid at the N-terminus is Lys, a C3 to C10 fatty acid may be further bound to an amine group,
- Tyr may be substituted with halogen, and
- Asn may be glycosylated.
An antibacterial peptide or a salt thereof, wherein the antibacterial peptide consists of any one amino acid sequence selected from the group consisting of Trp-Leu-Leu-Trp-Ile-Gly-Leu-Arg-Lys-Lys-Arg (SEQ ID NO:
- 24), Trp-Leu-Leu-Trp-Ile-Ala-Leu-Arg-Lys-Lys (SEQ ID NO:
- 25), Lys-Trp-Leu-Leu-Trp-Ile-Gly-Leu-Arg-Lys-Lys-Arg (SEQ ID NO:
- 26), Trp-Ala-Ala-Trp-Ile-Gly-Leu-Arg-Lys-Lys-Arg (SEQ ID NO:
- 27), Trp-Leu-Val-Trp-Ile-Gly-Leu-Arg-Lys-Lys-Arg (SEQ ID NO:
- 28), Trp-Leu-Ile-Trp-Ile-Gly-Leu-Arg-Lys-Lys-Arg (SEQ ID NO:
- 29), Trp-Leu-Phe-Trp-Ile-Gly-Leu-Arg-Lys-Lys-Arg (SEQ ID NO:
- 30), Trp-Leu-Tyr-Trp-Ile-Leu-Leu-Arg-Lys-Lys-Arg (SEQ ID NO:
- 31), Trp-Leu-Val-Trp-Ile-Tyr-Leu-Arg-Lys-Lys-Arg (SEQ ID NO:
- 32), Trp-Leu-Val-Trp-Val-Tyr-Leu-Arg-Lys-Lys-Arg (SEQ ID NO:
- 33), Trp-Leu-Val-Trp-Ile-Tyr-Val-Arg-Lys-Lys-Arg (SEQ ID NO:
- 34), Trp-Leu-Val-Trp-Ile-Tyr-Arg-Lys-Lys-Arg (SEQ ID NO:
- 35), Trp-Leu-Ile-Trp-Val-Tyr-Arg-Lys-Lys-Arg (SEQ ID NO:
- 36), Trp-Leu-Val-Trp-Ile-Tyr-Arg-Arg-Arg (SEQ ID NO:
- 37), Trp-Val-Val-Val-Val-Trp-Arg-Arg-Arg (SEQ ID NO:
- 38), Trp-Val-Val-His-Val-Val-Trp-Arg-Arg-Arg(SEQ ID NO:
- 39), Arg-Arg-Arg-His-Val-His-Val-Val-Trp-Lys (SEQ ID NO:
- 43), Trp-Leu-Leu-Trp-Ile-Gly-Leu-Arg-Lys-Lys-Arg-Tyr (SEQ ID NO:
- 65), Trp-Leu-Val-Tyr-Val-Trp-Leu-Arg-Lys-Lys-Arg (SEQ ID NO:
- 68), Ser-Trp-Leu-Leu-Trp-Ile-Gly-Leu-Arg-Lys-Lys-Arg (SEQ ID NO:
- 55), Trp-Val-Val-His-Val-Val-Trp-Arg-Arg-Arg-Asn (glycosylated) (SEQ ID NO:
- 59), Arg-Arg-Arg-Trp-Val-Val-Val-Val-Val-Trp (SEQ ID NO:
- 97), Trp-Leu-Leu-Trp-Ile-Ala-Leu-Arg-Lys-Lys-Arg (SEQ ID NO:
- 23), Trp-Leu-Leu-Trp-Ile-Gly-Leu-Arg-Lys-Lys (SEQ ID NO:
- 47), Leu-Leu-Trp-Ile-Ala-Leu-Arg-Lys-Lys-Arg (SEQ ID NO:
- 49), Leu-Leu-Trp-Ile-Ala-Leu-Lys-Lys-Lys-Lys (SEQ ID NO:
- 50), Leu-Leu-Trp-Ile-Gly-Leu-Arg-Lys-Lys-Arg (SEQ ID NO:
- 51), Tyr-Trp-Leu-Leu-Trp-Ile-Gly-Leu-Arg-Lys-Lys-Arg (SEQ ID NO:
- 64), Trp-Leu-Leu-Trp-Val-Tyr-Val-Arg-Lys-Lys-Arg (SEQ ID NO:
- 66), Trp-Leu-Trp-Val-Trp-Val-Arg-Lys-Lys-Arg (SEQ ID NO:
- 70), Val-Trp-Val-Trp-Val-Trp-Val-Arg-Lys-Lys-Arg (SEQ ID NO:
- 76), Trp-Val-Val-Trp-Val-Tyr-Val-Arg-Lys-Lys-Arg (SEQ ID NO:
- 71), Trp-Val-Val-Trp-Val-Val-Tyr-Arg-Lys-Lys-Arg (SEQ ID NO:
- 72), Trp-Leu-Ala-Trp-Leu-Tyr-Leu-Arg-Lys-Lys-Arg (SEQ ID NO:
- 73), Trp-Leu-Leu-Trp-Leu-Tyr-Ala-Arg-Lys-Lys-Arg (SEQ ID NO:
- 74), Trp-Ala-Ala-Trp-Ile-Gly-Leu-Arg-Arg-Arg (SEQ ID NO:
- 80), Trp-Ala-Ala-Trp-Ile-Gly-Leu-Arg-Lys-Lys-Lys (SEQ ID NO:
- 86), Trp-Ala-Ala-Trp-Ile-Gly-Leu-Arg-Arg-Lys-Lys (SEQ ID NO:
- 87), Trp-Ala-Ala-Trp-Ile-Gly-Leu-Arg-Lys-Arg-Lys (SEQ ID NO:
- 88), Trp-Ala-Ala-Trp-Ile-Gly-Leu-Arg-Arg (SEQ ID NO:
- 89), Arg-Arg-Arg-Trp-Val-Trp-Val-Val-Val-Trp (SEQ ID NO:
- 91) and Arg-Arg-Arg-Trp-Val-Val-Val-Val-Trp-Trp (SEQ ID NO:
- 96).
Dependencies are inferred from the claim text.