Immunostimulatory compositions comprising soluble parasite extracts and uses thereof
AssigneeGriffith University
InventorsMichael GOOD, Danielle STANISIC, Ashwini Kumar GIDDAM, Aloysious SSEMEGANDA
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Abstract
Disclosed are compositions for stimulating a protective or therapeutic immune response to an apicomplexan parasite such as those from the Plasmodium or Babesia genus. More particularly, the compositions comprise a soluble parasite extract. The extract may be free of red blood cell components and/or contained in or associated with a particle such as a liposome. The compositions and methods disclosed herein are particularly useful in the prevention and treatment of parasitic diseases.
Claims34
An immunostimulatory composition comprising a soluble parasite extract, wherein the composition is substantially free of insoluble parasite components or red blood cell (RBC) components.
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A method of preparing an immunomodulatory composition for eliciting an immune response to a parasite antigen, the method comprising:
- harvesting parasitized red blood cells (pRBCs);
- lysing the pRBC cells under conditions sufficient to lyse the membrane of red blood cells but not sufficient to significantly lyse the parasite membranes;
- harvesting the insoluble fraction of the pRBC lysate, wherein the insoluble fraction comprises red blood cell membranes and whole parasites;
- lysing the parasite membranes;
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- harvesting the soluble parasite fraction, wherein the soluble parasite fraction comprises soluble parasite antigens;
- to thereby produce an immunogenic composition sufficient to elicit an immune response to the parasite.
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An immunostimulatory composition comprising a whole parasite extract contained in or otherwise associated with a particle, wherein the whole parasite antigen component is substantially free or completely free of red blood cell components.
Dependencies are inferred from the claim text.