Target binding moiety compositions and methods of use
AssigneeRootPath Genomics, Inc.
InventorsXi Chen, Ely Porter
Life of this patent
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Abstract
Provided herein are compositions and methods to identify a binding element (e.g., peptide, peptoid, or protein) that can be bound by an immunoreceptor (e.g., antibody). The binding element can be provided in a target binding unit comprising two binding elements separated by a spacer such that the two binding elements simultaneously bind to a single molecule comprising an antigen binding domain of an antibody. The present disclosure provides various methods to construct the spacer. The identified binding elements can be further used to manufacture an array which can be used to profile antibodies obtained from a blood sample.
Claims21
-211. (canceled)
A method for manufacturing a polynucleotide barcoded target binding moiety, the method comprising:
- (a) translating an RNA sequence of an RNA molecule, wherein the RNA sequence encodes a peptide sequence, wherein the RNA molecule is linked to a peptide acceptor at a 3′ end of the RNA molecule;
- and
- (b) linking the peptide acceptor to an amino acid residue of a translated peptide comprising the peptide sequence, thereby forming a nucleic acid-peptide fusion molecule, wherein the nucleic acid-peptide fusion molecule comprises a polynucleotide barcoded target binding moiety comprising:
- (i) a first peptide sequence comprising a first binding region, and
- (ii) a second peptide sequence comprising a second binding region;
- wherein the first binding region and the second binding region are
- (i) separated by a spacer, and
- (ii) spaced at a distance such that the first peptide sequence and the second peptide sequence simultaneously bind to a single molecule comprising an antigen binding domain of an antibody.
Dependencies are inferred from the claim text.