# US 2023/0273418 A1 · Method and illumination device of the adaptive optics in transmission or reflection microscopy > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 2023/0273418 A1 - Document ID: US20230273418A1 - Status: Published application - Kind: A1 - Application number: 18/017,418 - Filed: 2021-07-19 - Published: 2023-08-31 - Earliest claimed: 2020-07-24 - Assignees: Max-Planck-Gesellschaft zur Foerderung der Wissenschaften e.V. - Applicants: Max-Planck-Gesellschaft Zur Foerderung Der Wissenschaften E.V. - Inventors: IVAN VISHNIAKOU; JOHANNES DOMINIK SEELIG - Web page: https://patentgrep.com/patent/US20230273418A1 ## CPC - G02B 21/0032: {Optical details of illumination, e.g. light-sources, pinholes, beam splitters, slits, fibers (G02B21/0036 - G02B21/008; means for illumination of specimens in general G02B21/06)} - G02B 21/008: {Details of detection or image processing, including general computer control} - G02B 21/06: Means for illuminating specimens - G02B 21/361: {Optical details, e.g. image relay to the camera or image sensor (G02B21/364 takes precedence; illumination details G02B21/06 and subgroups)} - G02B 21/365: {Control or image processing arrangements for digital or video microscopes (G02B21/361, G02B21/362 take precedence)} - G02B 27/0933: {Systems for active beam shaping by rapid movement of an element} - G02B 21/0076: {arrangements using fluorescence or luminescence} - G02B: OPTICAL ELEMENTS, SYSTEMS OR APPARATUS ## Abstract A method for optimizing parameters of a physical light propagation model includes providing a physical model of a light propagation in an optical system, radiating an input light distribution using an illumination unit into an excitation path of the optical system, traversing the input light distribution through a scattering body, wherein the scattering body is arranged in the excitation path of the optical system and modifies the input light distribution to form a transmission light distribution to form a reflection light distribution, recording the transmission light distribution or the reflection light distribution, transferring the recorded transmission light distribution or the recorded reflection light distribution to the physical model, and computing transmission distortion parameters of the physical model based on the recorded transmission light distribution or the recorded reflection light distribution. The transmission distortion parameters characterize the scattering body. ## Claims ### Claim at position 1 1-11. (canceled) ### Claim at position 2 12: A method for optimizing parameters of a physical light propagation model, the method comprising: providing a physical model Mlight of a light propagation in an optical system; radiating an input light distribution I0 using an illumination unit into an excitation path of the optical system; traversing the input light distribution I0 through a scattering body, wherein the scattering body is arranged in the excitation path of the optical system and modifies the input light distribution I0 to form a transmission light distribution ITR or I0 to form a reflection light distribution IRE; recording the transmission light distribution ITR or the reflection light distribution IRE; transferring the recorded transmission light distribution ITR or the recorded reflection light distribution IRE to the physical model Mlight; and computing transmission distortion parameters VTR of the physical model Mlight based on the recorded transmission light distribution ITR or the recorded reflection light distribution IRE, wherein the transmission distortion parameters VTR characterize the scattering body. ### Claim at position 3 (depends on 12) 13: The method as recited in claim 12, wherein, the physical light propagation model is a light propagation model in laser microscopy in a scanning method, and the light propagation in the optical system is a light propogation in a microscope. ### Claim at position 4 (depends on 12) 14: The method as recited in claim 12, wherein the recording of the transmission light distribution ITR is performed using a camera. ### Claim at position 5 (depends on 14) 15: The method as recited in claim 14, wherein the recording of the transmission light distribution ITR using the camera is performed at a virtual location of a sample. ### Claim at position 6 (depends on 12) 16: The method as recited in claim 12, wherein an n-fold transmission light distribution data set n-ITR is generated by an n-fold variation of the input light distribution I0. ### Claim at position 7 (depends on 12) 17: The method as recited in claim 12, wherein, the physical model Mlight is provided on a computer unit, and the computing of the transmission distortion parameters VTR of the physical model Mlight based on the recorded transmission light distribution ITR is performed automatically via an algorithm implemented on the computer unit. ### Claim at position 8 (depends on 12) 18: The method as recited in claim 12, wherein a mathematically differentiable model Mlight is used as the physical model Mlight of the light propagation. ### Claim at position 9 (depends on 12) 19: The method as recited in claim 12, further comprising: reflecting the transmission light distribution ITR at a location of the sample using a reflective material so that the transmission light distribution ITR again traverses the scattering body and is modified to form a reflection light distribution IRE; and recording the refection light distribution IRE. ### Claim at position 10 (depends on 19) 20: The method as recited in claim 19, wherein the reflective material is a mirror. ### Claim at position 11 (depends on 19) 21: The method as recited in claim 19, further comprising: computing reflection distortion parameters VRE of the physical model Mlight based on the recorded reflection light distribution IRE. ### Claim at position 12 (depends on 12) 22: An irradiation device for performing the method as recited in claim 12, the irradiation device comprising: a microscope comprising, an illumination unit which is designed to generate a light distribution I0, and an excitation path comprising a scattering body which is arranged in the excitation path, the excitation path being configured to guide the light distribution I0 to a location of a sample; a camera which is configured to record a transmitted light ITR; and a computer unit comprising an algorithm implemented thereon, wherein, a physical model Mlight of a light propagation in the irradiation device is provided on the computer unit, and the algorithm implemented on the computer unit is configured to compute transmission distortion parameters VTR of the physical model Mlight based on the physical model Mlight and the transmitted light ITR. ### Claim at position 13 (depends on 22) 23: The irradiation device as recited in claim 22, wherein, the illumination unit is a laser, and the transmitted light ITR is at the location of the sample. ### Claim at position 14 (depends on 12) 24: A method for correcting distortions of a light distribution during passage through an optical system which comprises a scattering body, the method comprising: extracting a transmission distortion parameter VTR as computed in claim 12; and setting a complementary distortion pattern VTR# on an optical modulator of an excitation path, wherein, the optical modulator is arranged in a beam direction before the scattering body. ### Claim at position 15 (depends on 24) 25: The method as recited in claim 24, wherein the method is performed in a laser microscopy in a scanning method during an irradiation of a sample. ### Claim at position 16 (depends on 24) 26: The method as recited in claim 24, wherein, in addition to extracting the transmission distortion parameter VTR, a reflection distortion parameter VRE is extracted by computing reflection distortion parameters VRE of the physical model Mlight based on the recorded reflection light distribution IRE, and a complementary distortion pattern VRE# is set on a further optical modulator in a detection path. ### Claim at position 17 (depends on 24) 27: An irradiation device for performing the method as recited in claim 24, the irradiation device comprising: a microscope comprising, an illumination unit which is configured to generate a light distribution I0, and an excitation path comprising a scattering body which is arranged in the excitation path, the excitation path being configured to guide the light distribution I0 to a location of a sample; an optical modulator arranged in the excitation path in a light propagation direction before the scattering body; and a computer unit which is configured to, control the optical modulator, to extract transmission distortion parameters VTR from the physical model Mlight of the light propagation in the irradiation device, and to set the optical modulator using a complementary distortion pattern VTR# correcting distortions of the scattering body. ### Claim at position 18 (depends on 27) 28: The irradiation device as recited in claim 27, wherein, the illumination unit is a laser.